Asked 30th May, 2022

Would trypsin cleave up a protein with many lysines and arginines? Would other stomach proteases cut any protein that contain respective cut sites?

If I wanted to introduce a foreign protein into the human stomach without it getting cleaved up by proteases such as trypsin, how would I do so?

All Answers (3)

30th May, 2022
Shin Murakami
Touro University California
They describe something related to your question. Best of luck.
30th May, 2022
Adam B Shapiro
Entasis therapeutics
You would have to enclose the protein in something to protect it, such as a capsule with an enteric coating, but the protein would still be digested in the small intestine. That is why protein drugs are always delivered by subcutaneous injection or intravenous injection.
31st May, 2022
Malcolm Nobre
Advanced Centre for Treatment, Research and Education in Cancer
Hello Chris Lee
You may introduce protective groups that will help prevent enzymatic degradation of the foreign protein.
Cyclization of foreign protein can increase its half-life by protecting it from the digestion of exo- and endopeptidases. Cyclization of peptides and proteins generate more rigid conformations, and it exhibits decreased susceptibility to enzymatic degradation.
You can also protect the foreign protein from proteases by covalently attaching it to a biocompatible polymer, leading to reduced immunogenicity, enhanced bioavailability, and enriched pharmacokinetic profile.
For more information, you may refer to the attached paper.

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