Would anyone have answers for these 3 questions regarding the creation of semilandmarks using TPS series?

I am a beginner in Geometric Morphometrics and I am working with archaeological artefacts. I have these 3 doubts in regards to semilandmark building on TPS:

1. before placing the LM curve in all specimens, do I need to put all the pictures in the same scale using in tosDig, like we do for LMs?

2. The idea is to place 2 LMs in each object and then 14 semilandmarks in the contour. By watching the tutorial I realized that I wouldn’t need to put the 2 landmarks first, but only draw the semilandmarks curve all around each specimen, because later all these points are going to be transformed into landmarks in tpsUtil anyways — is that correct?

3. After converting the semilandmarks in landmarks on tpsUtil, how do I slide the semilandmarks of my specimens using TPSRelw? Is it only after the sliding step that the file will be ready to be used normally on MorphoJ? Then the protocol is the same as usual? — running the GPA, building classifiers, covariance matrix, PCA, etc etc?

TPS

Geometric Morphometrics

Passive Cutaneous Anaphylaxis

Covariance

Renata Pedroso de Araujo

University of São Paulo

Hi Verónica Fernández-Navarro ,

Regarding your answer for my question #3:

3. For sliding semilandmarks I personally use other programms as Viwebox, where you can work in 2D and 3D and its tools are wider. After that proccess you can easily export your raw coordinates and analyze them wherever you want. I use MorphoJ for that, it´s easy for beginners like us and the tools are more than ok.

I used TPSRelw to do the semilandmark sliding, but I'm not sure how to save the file there in order to run it in MorphoJ. Do you happen to know it?