Will the chemicals in pH paper or strips contaminate the final product?

I am working on Per and polyfluoroalkyl substances (PFAS) and have been trying to calculate the recoveries of PFCAs( C4 to C12) from WAX cartridge (Oasis Brand). I prepared PFCAs mixture (10 ppb, 20 ppb, 30 ppb , 65ppb and even 90 ppb) and carried out solid phase extraction (SPE). I followed Draft method 1633 and UPLC-MS/MS was used for quantification.

I was unable to quantify PFBA after SPE. For other PFCAs, the recoveries were even higher than 150%.

P.S. I used Manual nitrogen evaporator to concentrate the sample after SPE using gentle nitrogen flow at constant temperature (35 degree Centigrade)

I'm working on determining the composition of algae after culturing it with wastewater.

I plan on using a lab-scale centrifuge for algae harvesting. What speed and time are suitable for a 40 ml algae culture?

Any protocol or procedure available will be helpful.

Thank you.

My university does not have Scopus subscription. Is there any way that I can get the access to database?

Hi colleagues,

We want to dissolve PTFE in a suitable solvent to develop new generation coatings; can you have any idea about it?

As I know, perfluorohexane can dissolve it, but is there anyone who uses any other solvent that can dissolve PTFE? (such as IKV SOL XF?)

Thanks a lot!

I have docked numerous ligands into my protein of interest, I would like to know how I can calculate the binding affinities between protein and ligands, or alternatively, how can the docking scores be converted to binding affinities?

Thank you in advance!

Hi everyone,

I need some help regarding how to carryout fluorescence measurements. I am a beginner in this area and I need someone to point me in the right direction. I am trying to prepare a composite of zinc oxide and a fluorescent dye (solvatochromic dye) for luminescent sensing applications. Our lab has a fluorolog-3 spectrofluorometer which has not been used for a long time and the solid sample holder is probably missing, although we hope to get one soon. But first, I need to be able to evalute the performance of my fluorescent composite with different solvents. Where I need help is this: how can I prepare my sample for fluorescent detection experiment? Do I suspend a known amount of the solid powder in each solvent and put it in a cuvette and measure? Or I suspend the powder in one solvent and added a calculated volume of other solvents one at a time for measurements? Thank you very much for your kind responses.

Hi,

I want to take fluorescence spectra of a solid compound that is totally insoluble in most of solvents.

what can i do to do the measurement?

thanks for sharing your ideas.

what solvent can be used to remove decanol in solution? I used to synthesis my product using decanol as starting material, how can i remove it to get pure product during solvent extraction? Thank you.

Even though I used column chromatography as my last option, the yield I got is not good.