Why indica cultivars show recalcitrance to genetic and tissue culture manipulations than japonica cultivars?

transgenic soil salinity resistant wheat cultivar

Seeds were grown in sterile condition, but nevertheless bacteria emerged from their roots. Thus sure the leaves taken from the seedlings contain it too.

Tomatoes especially get me crazy.

Did someone experinece the same?

Generally, the artificial manipulation of canopy gradients in form of management intervention in old growth tropical forest with scarcity of regeneration and its establishment of commercially valuable species response promptly with remarkable density of seedling and sapling in forest floor. The commercially valuable species like Shore robusta have high potential to regenerate and recruit under open canopy.

What ratios of seedling to sapling and sapling to adult trees in natural stand could indicate the healthy forest and sustainable regeneration?

For transformation purpose

I am working on transgenic rice. We have standarised the regeneration and transformation protocol for it. But I could see two Albino plants that are regenerated from calli. Can you please help me to short out the issue. For your kind information I want to mention that other plants regenerted with same media and hormone componets including same light condition have not shown such abnormalities. Please find the attached file.

I just bought MS liquid medium, micro and macronutrient separately. I wont use the whole medium on a single time, so I must open it several times. My question is, should I manipulate it inside the cabinet, usign sterile glassware or can I use it outside? I obviously will sterilize my flasks when they are ready, but I need to know if the stock media can get contaminated? Besides, I have another question... one of the media changed to a light yellow color. is that contamination?

Thank you!

Shall we have previous genetic variation in explant tissues used in in vitro approaches, how can we estimate an adequate ratio for somaclonal variation among regeneration events?

In tissue culture we use several plant tissues and “reprogram” the cell development to obtain somatic embryos, calluses, shoot meristems, whatever.

Considering that the raw material we start with would not have a chance to follow another developmental sketch (E. g. a spongy parenchyma cell) is expected to die as originally planned, a spongy parenchyma cell.

It is clear that some stress triggers, frequent cell divisions, growth regulators and other substances, may increase the mutation ratio differently. We may also consider that there is a tiny probability of this mutation events occur by chance.

My reasoning is, how can we correctly attribute a somaclonal variation ratio being aware of the possibility of mutations that would not jeopardize a cell developmental path already settled in an explant (a chlorenchyma cell for instance) that, was not for the tissue culture detour, would “kick the bucket” as such (a chlorenchyma cell)?

Dear All,

Any experienced person in sweet orange tissue culture please tell me which explants will be more responive and productive for in vitro regeneration of sweet orange plant? Thanks in advance.