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Hello,

I am using the Qiagen PCR clean-up and purification kit. I am purifying a PCR product of 750 bp.

I have added the buffer PB as described in the manual. The color of the mixture was violet (too high pH) so I added 10 microliters of 3M Sodium acetate (pH 5.0) as advised by the manual. However, the mixture remains pinkish in color.

is there any other way to regulate the pH and lower it?

Hello everyone,

I am trying to generate a faulty acceleration signal with SIMULINK. The inner ring of the bearing is fixed to the shaft. The bearing has 17 rolling elements. I was thinking of creating a fault in the inner ring because it is attached to the shaft. My approach was to add 17 impulses per cycle to the original measured acceleration data in order to generate a faulty signal.

I attached a picture of my Simulink model. What do you think about this approach and is my model correct so far?

10.848 * f_wheel is the Ball Pass frequency of the inner ring.

Kind regards

I would like to know the advantages in terms of complexity in the analysis (which software is easier to use). Also, I want to know wich software is more reliable for the analysis of mechanism.

In order to develop application programs for analysis and monitoring of mechanical vibrations for condition monitoring and fault prediction, we need to analyze large, diverse datasets and build and validate models. How to obtain such datasets in the first place?

Please help perform this gene expression study using RT2 profiler qPCR array from qiagen and verified data using RT-PCR with 4 designed primers from a different manufacturer. My problem is, the fold change for the gene of interest in qPCR array was different from the fold change that I obtained in the RT-PCR technique. How do I explain the difference between the two fold change? Can i say it is due to the primers from two different manufacturers? Desperately need some answers!!

To prepare one report on the available biotechnological products/drugs in India i need to refer some patented articles. please help me with the links or the websites to find out the available patents. Thank you

Hello,

This is a question about actual usage of ViSQOL technology for end-to-end voice and audio quality analysis. One can find many papers regarding ViSQOL, but does anybody know companies that are actively using it? To my knowledge there are three technologies available on the market: ITU-T P.863 standard (POLQA), ViSQOL (initiated by Google) and Sevana AQuA. POLQA is widely used by many companies being an international standard. One can learn a bit about AQuA customers from Sevana website, but how to learn about the companies that are using ViSQOL?

Thanks a lot in advance!

Valeri