Question
Asked 4th Jan, 2023

Which solvents should I use to test antioxidant activity of polysaccharides using DPPH assay?

I have some fractions of polysaccharides and I want to test them by using DPPH. In general, people use methanol or ethanol as a solvent to dissolve DHHP. But my samples can precipitate with them

Most recent answer

9th Feb, 2023
Palpandian Preethi
Indian Council of Agricultural Research
We are using 80% methanol

Popular answers (1)

4th Jan, 2023
Abdelkader BOUAZIZ
SOUGUEUR Division - Chemistry (POLYMERS), Université Ibn Khaldoun Tiaret
Dear Yen Thi My Nguyen, solubility is not of sole importance, though methanol is the most casually used solvent, other consideration on the influence of the solvent on the quality of analysis. Please have a look at the following links. My Regards
9 Recommendations

All Answers (4)

4th Jan, 2023
Abdelkader BOUAZIZ
SOUGUEUR Division - Chemistry (POLYMERS), Université Ibn Khaldoun Tiaret
Dear Yen Thi My Nguyen, solubility is not of sole importance, though methanol is the most casually used solvent, other consideration on the influence of the solvent on the quality of analysis. Please have a look at the following links. My Regards
9 Recommendations
9th Jan, 2023
Md. Sakhawot Hossain
Jashore University of Science and Technology (JUST)
The most common solvents used to test the antioxidant activity of polysaccharides using the DPPH assay are methanol, ethanol, and acetone. In addition, water, dimethylsulfoxide (DMSO), and dimethylformamide (DMF) may also be used.
3 Recommendations
13th Jan, 2023
Rohit Thirumdas
Professor Jayashankar Telangana State Agricultural University
Methanol
9th Feb, 2023
Palpandian Preethi
Indian Council of Agricultural Research
We are using 80% methanol

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