Which type of insulin should be used to carry out Insulin Tolerance Test in type 2 diabetic rats?

NAFLD shares many overlapping features with the insulin resistance syndrome, also known as "Syndrome X" including central or visceral obesity, type II diabetes, and dyslipidemia. Of course, NAFLD is now recognized as a hepatic manifestation of insulin resistance syndrome.

Dear esteemed colleagues,

Does anyone have any insight why most of the neuroendocrine studies, i.e hypothalamus-pituitary-peripheral organs axis, are mostly studied in mice? I do know some studies were done in rat, and even there are some rat animal models (HFD, genetic models, streptozocin, etc) but to my knowledge this is overwhelmingly minor compared to mouse models.

Is there any particular reason, such as mice neuroendocrine system more closely mimics human's? Or is there any technical reason? Thank you!

i am working on TEA as a substrate for OCT2 in proximal tubules to determine the OCT2 activity in-vivo.. i am injecting TEA i.v and then take blood samples to determine TEA concentration in serum .. i want a recent simple procedure that can be detected spectrophotometrically 

We are doing a longitudinal study on the tongues of lean and obese zucker rats. I wanted to study the gene expression of certain fat transporters in the tongue to see how they vary in lean/obese zucker rats as they gain weight over time.

Anyone have any ideas/publications to help? I have come across CD36 and GPR120 as possibilities, as well as Glut4. Any insight would be greatly appreciated

I found that lower insulin levels were associated with higher lactate levels.

In our previous studies, we found that the gluconeogenic genes (G6Pase and PEPCK) were down-regulated in KO rats compared with WT controls. Consequently, we performed the pyruvate tolerance test to examine the hepatic gluconeogenesis. However, when I injected 2 g/kg pyruvate (a stock solution of 20 % sodium pyruvate in sterile PBS, the pH of the solution was not adjusted because Na-pyruvate will not change the pH significantly) into SD rats, but they contorted their body into painful postures and 30 minutes later died. Actually, the concentration of pyruvate in my work is much higher than the physiological osmotic pressure of the rats. I also tried a lower concentration, but the change of blood glucose level was small. How could I solve this problem? Would anyone give me some suggestion or cues when performing this experiment?

am working on diabetic rats via STZ administration intraperitoneally.

can anyone suggest how can i mimic type 2 diabetes? do i need to give

fat diet to induce obesity?