What tubing material is the best in terms of biocompatibility for cell culturing?

I couldn't see much paper where plant breeders use biochemical such as proline content Malondialdehyde (MDA) and dyes such as NBT or DAB( for ROS detection) for screening stress-tolerant accession on a large scale (100-200 which I suppose is possible to do). Are not these methods better than phenotyping grain yield, biomass, plant height, NDVI, LAI, etc?

I will start an SSF for fish and I want to know what is the best inoculum size to start with ? Is that different from one bacteria strain to another?

What are the limitations and disadvantages of Real-Time PCR (RT-PCR)?

What is a more specific and sensitive technique that can be used in the laboratory instead, particularly in cancer diagnosis?

In other words, the production of true seed from the self-pollination of in vitro-derived haploid plant is possible or not?

I am verymuch intreasted in doing research in the field of the Additive Manufacturing technology with 3D printing process. Because of that i would like to know about the clear details of the present updates and future trends in the field of the Additive Manufactuirng.

I am egarly wait and intreaated for the collobirative research work in the field of Additive Manufactuirng with 3D printing process.

Dear colleagues,

I am writing to inquire if anyone knows how and where to find some unused antibody that was discontinued by Santa Cruz Biotechnology (KCNQ1 Antibody (C-20): sc-10646). Our lab would like to use it to complete some previous results that we obtained with that particular antibody. SCBT has an alternative antibody as a replacement, but we would prefer to use the old one if possible.

If anyone has any remaining stock of this antibody, or if you know of a source where it may still be available, please let me know. Your assistance in this matter would be greatly appreciated.

I'm trying to thiolate Poly(Acrylic acid) (PAA) with L-cysteine using EDC (or EDAC), however prior to the addition of L-cys, when I add EDC to PAA large clumps form in solution.

1g of PAA was dissolved in 100ml of deionized water, and 2ml of EDC was dissolved 10ml of water. The pH of both solutions was adjusted to approximately pH 5. The EDC solution was added dropwise to the PAA dropwise over 30 minutes, with stirring. The polymer started to clump together upon the addition of the EDC and failed to dissolve fully. Any suggestions as to why this may be happening?

We are looking for any software that is used in water absorption kinetics modeling like Peleg's model, Mohsenin's model and so on.