What is the true definition of gels?

Actually I'm working with colloidal dispersion gels, I want to know what is the best process for sample preparation for electron microscopy and which microscope could be used, tem or sem?

hello, I have received  the  message, considering the project: Advenced structural characterizations of clay minerals, related materials and ceramicsha s this project  any stable  financial  support  or  not?

Dear Guoping, I see that you cited one of my transaction papers in a new project for you. Do you have published new paper concerning the finding of W max/Min? If yes, please send me the reference to download the paper. Thank you in advance. Regards. Ali Chamas Al Ghouwayel. 

to run 2-D gels, minimum required cancer tissue, because in papers they did not mention tissue quantity. Thanks

Marker bands are not properly resolved when the gel run is complete. There are only 5 bands instead of 6 bands.

But when i pre run the gel for some time and loaded the same marker, all 6 bands were resolved as well as marker bands were crisp too.

What can be the reason apart from pre-running the gel?

Dear Basiliyos,

Greetings, It is a nice project. could  you elaborate me a bit, the main scientific  gaps currently investigated by you and your future contribution to fulfill this scientific gaps in related to your field of specialization,

Thanks my bro.

Regs,

Derese 

plz recomend methods

i have a problem about Mixing in pipe(polymerization).

because of polymerization speed(too fast), static mixer used to be blocked with gel(polymer gel).

so i need pre-mixing front of static mixer.

i thought about method

mixing plate (make turbulant flow) -> raw material's viscosity is high (X)

jet mixer -> can make a gel (fast polymerization) -> can be block the injector hole

so to solve a problem plz recomend any method which have a small possibility !

thank you

Hi,

I am running 10% Bis-Tris gel to check if my DNA-protein cross-link works. Lately i have problems with my coomassie staining. After staining with coomassie i noticed that no bands were showing up while I know that there is plenty of protein in it.

I stained it before without fixation and it was always working. I changed out the coomassie just in case.

Does anyone has an idea what is going on? All the advise will be appreciate it!

Thanks

Johannes

Wondering if anyone has any insight on why my gels are not running well? They are bowing in the center when running and it constantly switches itself off (the power cell will say that the load is not detected but it had been running just fine for 10-15 min).

Notes: It is the bio-rad mini protean system. I have checked all of the parts and when I run checks with precast gels they run perfectly (they are older gels and the percentage is not correct so I do not use them). The only problem I have is when I run my own handcast gels. I have been making gels for years with no issues and I have the same recipes that have worked well for me. When I run my own gels with the samples I have (similar samples run just fine on my other equipment), the gel bands bow and the powercell constantly throws an error.

Buffers are brand new and made fresh every time. All equipment is cleaned between uses. I have worked hard to see if there are any leaks with my gels. I run at 100V until the bands have run to the bottom. The samples are not high in any salts or urea. Gels do not have any bubbles.

Any ideas why my gels (that have been fine everywhere else) are not working here? Any tips? Thanks so much!