Plz refer the following links. Hope this should help
are tris Cl and tris HCl are same?
i think it is just the usage of PH dependent buffer solution. HCl makes the Tris PH value under 7. I hope this could help....
For Tris-base you add HCl for adjusting pH
most of the times I prepare tris buffer and adjust to ph 7.2.. after filtration I have observed increase in ph of about 0.2 to 0.3.. how can I come over with this problem?
Lalan Kumar Arya
Madurai Kamaraj University
Florida International University
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Korea Advanced Institute of Science and Technology
Central Manchester University Hospitals NHS Foundation Trust
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Emre Can Tüysüz
Federal University Wukari, Nigeria
Centre for Cellular and Molecular Biology
Samir Al Jbour