You definitely must try disk assay first. After screening with agar disk diffusion assay,
you can then evaluate the activity more with agar well diff. assay.
I think disk diffusion is much better, it should be followed by MIC and MBC tests.
If you could perform time-kill test after that, it will strengthen your study.
S. Zafar Haider
Centre for Aromatic Plants (CAP), Industrial Estate Selaqui-248197, Dehradun (Uttarakhand) INDIA
Centre of Advanced Study in Marine Biology
Michael John Dempsey
Manchester Metropolitan University
University College Cork
Jawaharlal Nehru Technological University, Hyderabad
Chemistry & Chemical Engineering Research Center of Iran
Emad M. Abdallah
Khorasan Institute of Higher Education