I am measuring chelating activity of microbial extract using ferrozine-Fe2+ complex formation. According to literature, control should be devoid of microbial extract (which has colour). Due to this, my control OD is less than test OD. How to solve the problem Please help.
Measuring total thiol is the significant way of calculating total antioxidant capacity.
I also use DPPH assay.
I Think Dr. Tresina has rightly answered your question and that too in a very elaborative way.
Kirori Mal College
CSIR - Institute of Himalayan Bioresource Technology
Government Meera Girls College
Tabriz University of Medical Sciences
Luis F Guido
University of Porto
S. P. Koh
Malaysian Agricultural Research and Development Institute (MARDI)
Veerabahu Ramasamy Mohan
V.O.Chidambaram College, Tuticorin
Visvesvaraya National Institute of Technology
Romina Lasagni Vitar
University of Buenos Aires