Talking about Sodium citrate, should be carrefull about the % of molarity ! when it comes to platelets, it is better to use 3.2% (0.105-0.109 M/L) - GEHT 2007. Further more, you should use an intact tube (non already used for coagulation) reagrding loss volumes (PT, APTT,Fib,...) and as always add around 10% on the final count
Can anyone share their experience with Sarstedt ThromboCount?
Some laboratories in France continue to use rarely UNOPETTE for PLT count. Not at my lab. Regarding PLT agregates on smear, Citrate 0.109 3.2% tube is demanded and PLT count is done (automatically added 10% by the Automate). The PLT contrôle count is however done in all patients facing Thrombocytopenia, where is clumps or not.
My preference also goes for Na-citrate.
when you are using Socium Citrate (blue top) anticoagulant tube for counting platelet clumps appearing in the EDTA tube (purple top), you should have the floor draw both the blue and purple tops. Then you have to multiply the platelet count obtained from the blue top by 1.1 to derive the correct platelet count.
If here is thrombocytopenia and confirm on blood smear and see platelet clumps if blood sample collected in EDTA anticoagulant (Facticious or pseudo thrombocytopneia), ask for collect sample in either Sodium citrate or in heparin.If in citrate need correction (10% ).
You should advise to collect both the EDTA and Sodium Citrate tops for this platelet clump problem. Rum them both with your automted hematology instrument. You should be able to still see the platelet clumps on the smear made from the EDTA tube specimen, but no clumps on the smear made from the socium citrate top tube specimen. Then apply the dilution factor of 1.1 to the platelet count from the sodium citrate top to obtain the accurate platelet count, i.e. multiply the platelet count result from Sodium Citrate specimen by 1.1 to derive the true platelet count.
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