What barriers are there for utilizing and accessing treatment for opioid use disorder in the Native American/Alaskan Indian population?

We are trying to stain neutrophils in the neonatal hypoxic-ischemic brain. We tested two markers, NE and MPO, but both showed signals not only in neutrophil-like cells (based on morphology) but also in neurons and oligodendrocytes. When we used the same method and concentration on adult stroke brain samples, we observed very specific neutrophil staining.

Does anyone know how to interpret this? The samples we used were FFPE sections, and the processing, sectioning, and staining methods for both neonatal and adult brains were identical.

The antibodies showed very specific signals in the adult brain, indicating that they work well. However, why do they show so much nonspecific staining in neonatal brains? Do we need to use a different method to process and stain neonatal brain tissue?

Thank you very much for any suggestions.

Chunfeng

Hi everyone,

We want to do some hippocampal volume measurements on Nissl stained coronal sections from mouse brains. We want to divide those measurements, distinguishing between dorsal, intermediate, and ventral parts of the hippocampus to evaluate changes in volume related to affective states and chronic stress (previous studies suggesting that ventral part is more sensitive than dorsal).

However, we can't seem to find a standard peer reviewed approach to measuring dorsal / ventral hippocampal volume in mice. Anyone have some advice? Or are willing to share their methods/materials with us?

Thanks for your help!

Soy estudiante de medicina y busco identificar cómo estas dimensiones contribuyen al trastorno y cómo un enfoque terapéutico que integre estos factores podría mejorar la efectividad del tratamiento.

Time restricted eating/feeding has become of major interest to the treatment of neuroinflammation in Alzheimer’s disease patients. The effects of time restricted eating/feeding on gut microbiome is now important to neuroinflammatory process and brain amyloidosis. Bacterial contaminations with gram negative bacteria is associated with the generation of toxic lipopolysaccharides (LPS) structures that cause inflammation, hypercholesterolemia and amyloid beta aggregation with relevance to neurodegeneration. The understanding of the role of time-restricted eating/feeding, gut microbiota and LPS induced neuroinflammation and the metabolic regulation of amyloid beta is now important to the induction of Alzheimer’s disease.

RELEVANT REFERENCES:

1. Maha Gasmi, Novi Silvia Hardiany, Marie van der Merwe, Ian J. Martins, Aastha Sharma & Ruth Williams-Hooker (02 Jul 2024): The influence of time-restricted eating/feeding on Alzheimer’s biomarkers and gut microbiota, Nutritional Neuroscience, 2024.

2. Sharma, A and IJ Martins. The role of Microbiota in the pathogenesis of Alzheimer’s disease. Acta Scientific Nutritional Health. 7.7 (2023): 108-118.

How Medications Can Affect Dental Implant Treatment

Hello everyone.

I am attempting to do visual cortex imaging in mice anesthetized with halothane, but the survival rate of these mice is quite low, even compared to other mice anesthetized with halothane.

I realized the batch of halothane in my lab is quite old, and I'm trying to find out if it's the reason why the mice are not surviving long enough, or whether it's viable to use. Calling the manufacturing company did not help.