Question
Asked 3 October 2013

What are saponins and what are their health benefits?

Certain beneficial effect of saponins such as lowering of plasma cholesterol levels in humans, anticarcinogenic activity, inhibitory effect on infectivity of HIV in vitro, antioxidant activity of saponins and protective effect on liver injury. Considerable quantity of soyasaponins are reported in soybean and beans. Soyasaponins are classified into two major groups, soyasaponin A and B. Group A acetylated saponins present in soybean are mostly responsible for undesirable bitter and astringent taste, whereas Group B saponins possess several health benefits. Recent in vitro studies have established that the health benefits such as hypocholesterolemic (cholesterol lowering) effect, anti-carcinogenic, anti-oxidative, anti-tumor, anti-virus, anti-hepatic, anti-diabetic and hepato-protective properties of food legumes are due to presence of group B saponins. Dietary saponins of soybeans are advantageous in preventing hypercholesterolemia and aortic atherosclerosis.

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Denise Goodfellow
Southern Cross University
Thank you Dr. Farhang.
Regards,
Denise Goodfellow

Popular answers (1)

Arvind Singh
Banaras Hindu University
Saponins are naturally occurring surface active glycosides mainly produced by plants, besides some bacteria and lower marine animals. Their structure consists of a sugar moiety linked to a hydrophobic aglycone called sapogenin. The sugar moiety may contain glucose, galactose, rhamnose, methylpentose, glucuronic acid or xylose, while the aglycone portion may be a steroid or a triterpene. Steroidal saponins are mainly abundant in monocotyledons, while dicotyledons predominantly contain triterpenoid saponins. Saponins may help reduce cholesterol levels, strengthen the immune system, treats diabetes, and inhibit tumor growth. They also improve lipid metabolism and may help prevent and treat obesity. Please see the following RG links for more details.
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All Answers (10)

Esam H. Mansour
Faculty of Agriculture, Menoufia University, Shibin El-Kom, Egypt
Saponins are phytochemicals which can be found in most vegetables, beans and herbs. The best known sources of saponins are peas, soybeans, and some herbs with names indicating foaming properties such as soapwort, saoproot, soapbark and soapberry.
 
Saponins are glucosides with foaming characteristics. Saponins consist of a polycyclic aglycones attached to one or more sugar side chains. The aglycone part, which is also called sapogenin, is either steroid (C27) or a triterpene (C30). The foaming ability of saponins is caused by the combination of a hydrophobic (fat-soluble) sapogenin and a hydrophilic (water-soluble) sugar part. Saponins have a bitter taste. Some saponins are toxic and are known as sapotoxin.
Health Benefits of Saponins
Saponins cause a reduction of blood cholesterol by preventing its re-absorption.
Saponins have antitumor and anti-mutagenic activities and canlower the risk of human cancers, by preventing cancer cells from growing.
Saponins also seem to help our immune system and to protect against viruses and bacteria. 
Saponins have a protective role on bone loss. 
 
The non-sugar part of saponins have a direct antioxidant acitivity, which may results in other benefits such as reduced risk of cancer and heart diseases.
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Miikue-Yobe Togenu
Francis Laboratories, c/o SLT Dept, Rivers state Polytechnic
study has also shown that saponins are the active ingredients in certain herbs such as heinsia crinata that mediate the antidiabetic activity of the herb.
Denise Goodfellow
Southern Cross University
Saponins are found in some plants used by Aboriginal people for their medicinal properties, for example, Acacia holosericea, A. auriculiformis, and Alphitonia excela.
Arvind Singh
Banaras Hindu University
Saponins are naturally occurring surface active glycosides mainly produced by plants, besides some bacteria and lower marine animals. Their structure consists of a sugar moiety linked to a hydrophobic aglycone called sapogenin. The sugar moiety may contain glucose, galactose, rhamnose, methylpentose, glucuronic acid or xylose, while the aglycone portion may be a steroid or a triterpene. Steroidal saponins are mainly abundant in monocotyledons, while dicotyledons predominantly contain triterpenoid saponins. Saponins may help reduce cholesterol levels, strengthen the immune system, treats diabetes, and inhibit tumor growth. They also improve lipid metabolism and may help prevent and treat obesity. Please see the following RG links for more details.
5 Recommendations
Munish Sharma
Central University of Himachal Pradesh
Saponins are strongly bitter-tasting, surface active compounds with a structure consisting of a steroid or triterpenoid aglycone and one or more sugar chains, which are present in quinoa seeds at significant levels.
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Sura Aldewachi
Ninevah University
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Murwan khalid Sabahelkhier
Faculty of Science and Technology, Al-Neelain UniversityAl
Saponins are naturally available compounds that are found in all cells of legume plants. Chemically, saponins contain a carbohydrate part linked to a triterpenoid or steroids.
Benefits of saponin:
1. Reduce risk of cancer
2. Lowering cholesterol level
3. Reduce blood lipid
4. Lowering blood glucose level
5. It is used for inhibition dental caries and
6. It is used for inhibition platelets aggregation
7. It is used as antidote against acute lead poisoning.
Hamid Reza Farhang
Isfahan University of Technology
You could study the following link to get general/overview information about the saponin compound.
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Denise Goodfellow
Southern Cross University
Thank you Dr. Farhang.
Regards,
Denise Goodfellow

Similar questions and discussions

What am I doing wrong - extracting a sapogenin for NMR?
Question
3 answers
  • Jamie LoveJamie Love
I use hydrochloric acid to release a steroidal sapogenin from its saponin, then neutralise it with sodium bicarbonate. I am confident the hydrolysis works because the mixture no longer produces a persistent foam.
I know, from the literature, that this sapogenin is poorly soluble in water but very soluble in chloroform, so I phase extract the aqueous mixture with chloroform. I send the chloroform layer to my collaborator who evaporates it and resuspends in deuterated chloroform to do NMR. I expect several milligrams of the sapogenin but he has been unable to detect any. I'm losing my sapogenin. What am I doing wrong?
Some details and observations:
1) I add 2mls of concentrated (10N) hydrochloric acid to 8mls of the saponin which is in 45% ethanol (it's a “tincture”) so gets diluted to 36% ethanol when the hydrochloric acid is it added.
2) This (10ml) 2N reaction is incubated for 4 hours at 80C in a sealed polypropylene tube.
3) After incubation, the entire 10mls is poured into a flask containing 3 grams of sodium biocarbionate and mixed. Once the foaming (caused by release of carbon dioxide) has stopped, I pour the material into a new polypropylene tube. A lot of salt (NaCl) is produced from the neutralisation step. This salt and unreacted sodium bicarbonate settle to the bottom. I assume the solution is saturated with sodium ions.
4) Copious amounts of a dark red precipitate forms – even under conditions using much less acid, such as 0.01N reactions. When the solution is spun in a table top centrifuge, it produces a pellet that is about 1.5ml (on top of the salt formed by neutralisation) from 10mls of solution. The supernatant (from this 0.01N reactions) still produces a persistent foam. The red pellet, suspended in water, does not.
Not surprisingly, reactions at 2N also produce this precipitation but it is much darker – like a dark purple approaching black – and there is no persistent foam.
I don't know what this stuff is but I'm guessing it's polytannins.
5) I add 4 mls of chloroform to the (hydrolysed and neutralised) solution. Seal the (polypropylene) tube and shake vigorously for 30 seconds. I then spin the tube in a table top centrifuge at 4000RPM for 5 minutes. Next I use a graduated plastic (polypropylene) transfer pipette to penetrate the aqueous layer, bubbled out the tip (which has a “plug” of “polytannins” and some aqueous) and then collect the chloroform from the bottom, while avoiding the salt pellet. I transfer this into a glass (scintillation) vial. Then I add another 4mls of chloroform and extract again. I end up with more than 8mls in the vial because some of the ethanol moves into the chloroform. (They're miscible.)
Attached is a pdf with some photos and experimental flow chart.
Thanking you in advance for your suggestions.
Best regards,
Jamie

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