Asked 6th Mar, 2020

What is the best cell membrane marker for live cell imaging?

I would like to know if someone has experience using cell membrane markers for live cell imaging.
I have tried with WGA AF680 combined with Hoechst and my results are not convincing. I cannot see the membranes really good... and I found an enormous bleaching.
I also have in my lab DRAQ5... so, I don't know if I should try with another red marker or if it would be better to combine DRAQ5 with a blue cell membrane marker.
Could you tell me your opinions and experience with this?
Thank you very much

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