What are the health and medicinal benefits of Ginger?

I mostly do voltage clamp recording using caesium methanesulfonate based low Cl- internal solution. I use a Narishige vertical puller to pull my electrodes. I constantly get a pipette resistance of 4.5-5 MOhm with my settings. Recently, another member of my lab started doing current clamp experiments and he is using K-gluconate internal, and he told me he found the resistance of his electrodes with my setting to be very high, around 11-12 MOhm. I was curious and I tried the potassium internal in my setup and it seemed to be correct, I found the resistance to be around 11. However, when I used caesium internal the resistance was still around 5 MOhm. I am wondering why this is the case. It is possible that maybe conductance varies between internals so there can be slight differences, but this is more than two-fold. I am wondering why this is the case? What should I consider my actual electrode resistance to be? When I patch, I have found the tip to be of perfect size for patching a striatal MSN, however I am unsure of the tip diameter.

Hi everyone,

I am interested in voltage gated sodium channel currently, and I would like to ask some question to you.

As it is usually known, voltage dependency of multiple ion channels including VGNCs depend on the S4 transmembrane voltage sensor domain.

What I wonder here is, is it possible to decouple the voltage sensitivity with ion channel gating by mutating the S4 VSD?

Though Chahine et al. found that the gating membrane potential can be shifted to more positive (J. Membrane Biol. 201, 9–24 (2004)), the activity still depends on voltage.

In addition, some of ion channels with the S4 VSD are also multimodal, that is, they can be activated by other stimulation, e.g. mechanical stimulation in Nav1.5.

Thus, another question is, do you think its possible to reverse hierarchy channel activation mechanism, like making mechanosensitivity to be dominat on Nav1.5 activation.

Thank you for reading!

Simple mixed-mode oscillations are the bursting oscillations are the combination of larger period oscillations and smaller period oscillations, is there any analysis method to identify them in the time series?

1-not diluted the blood and adding equal volume to the Ficoll separartion medium.

2- diluted the blood with pbs and added different volume of the Ficoll.

#Ficoll-Paque

#ficoll

#Lymphocyte_seperation_media

#PBMCs

Recently I built a LiNi0.5Mn1.5O4/Graphite full-cell (coin-cell) and found that the coulmbic efficiency is increasing with cycling which is quite abnormal trend. What can be the possible reasons for this?

I want to perform multi-input multi-output regression analysis. Can you suggest some tutorials?

Help will be appreciated 🙏

The Discovery of antidepressant natural products is an interesting task. Various classes of bioactive compounds are responsible for antidepressant potential.

Citespace or Vosviewer or Histcite or BibExcel, or CitNetExplorer.

which one of these you use / recommend?

and why?