What are the health and medicinal benefits of Ginger?

How i prepare stock solution from plant extract for antifungal activity my desired volume is 10mg/ml and total volume of plant extract is 19mg/ml please recommend any link of article kindly any body tell me about this solution

I am currently extracting DNA from plasma samples of HBV-positive clinical cases using the QIAGEN Mini Kit (250), following the manufacturer’s protocol step by step. Despite adhering strictly to the procedure from lysis to elution I consistently obtain very low DNA concentrations, as verified by both Nanodrop and fluorescence-based assays.

I have experimented with elution volumes of 200 µL and 100 µL, but the yield remains low. Could this issue be related to the performance or compatibility of the kit with my sample type? Alternatively, should I consider switching to a different kit or extraction method?

Any suggestions on optimizing the protocol or recommendations for alternative kits better suited for HBV DNA extraction from plasma would be greatly appreciated.

Thank you in advance for your insights!

Im doing biochemical analysis (phenols, flavonoids, and antioxidant) for wheat seeds, after i did all the steps i measured the absorbance of samples using spectrophotometer, so the question is how we can calculate the content of GAE for phenols, or quercetin for flavonoids in mg/g sample from the Abs?

Or how we can convert from abs to mg/g for both phenols (gallic acid standard) and flavonoids (quercetin standard)?

I saw in an article that the prepared plant aqueous extract was filtered with a suitable filter paper and then diluted to a certain extent and used directly in experiments. Of course, it is normal to work this way. However, is it correct to express the results in μL/mL? However, how could they have calculated how much to dilute the aqueous extract since it was used directly in the experiment, because a stock solution was not prepared since the extracts were not in powder form?

I also saw such a procedure in another study: "2 g of the plants were weighed and 100 ml of distilled water at 100 ° C was added to them and left to infuse for 10 minutes. After the infusion process was finished, it was filtered through filter paper and the supernatant part was stored at 4 ° C for use in experimental studies." However, while doing the experiments, how could this liquid extract be prepared in concentrations of "1 ml of plant extract with concentrations of 50, 250, 500, 750 and 1000 μg/μl" and 20 µl of plant extracts (0, 10, 50, 250, 500, 1000 µg/ml) in these ratios? Because this extract is in liquid form, so in order to dilute it in µg/ml, doesn't it need to remove the solvent of the extract and turn the extracts into powder?

I am asking these questions because I will be conducting a study on herbal teas and will be using the tea extracts I have prepared in liquid form. Therefore, I need to know the correct method for their use.

To make an alginate-polyvinyl alcohol-gelatin hydrogel scaffold through 3D printing in bone tissue engineering, how much of each polymer should we use so that in 3D printing, this scaffold has good viscosity, no bubbles and It comes out well from the 3D printing needle?

AAZ

So I am doing a solid phase extraction for the first time using hexane and Methyl tert butyl ether. While I can mix, vortex and separate in glass tubes, I do not have the glass centrifuge facility currently.

Can I use falcon tube for the centrifugation duration or the organic solvents will cause a problem for the SPE?