Tips on purification os carbon quantum dots by centrifugation?

I understand that in SEC, larger molecules or particles do not enter within in the pores and thereby elute faster with the void volume. However, there certainly have an upper limit of the particle size than can elute with the void volume, not get completely stuck due to their too big size.

The instrument works perfectly, but when the EG concentration is changed to 45 µM @21 min, the conductivity increases sharply? If the EG concentration is set at 10 µM, the conductivity remains stable with a perfect baseline; however, the sulfate peak exhibits a very small area

The PVA and drug was synthesized using solvent evaporation and nanoprecipitation. In this method, the drug was solubilized in ethyl acetate. The PVA was dispersed in water. Under constant stirring and application of heat, the PVA was solubilized in water. After complete dissolution of PVA in water, the drug dissolved in ethyl acetate was added to the PVA mixture drop by drop under continuous stirring. Once the drug dissolved ethyl acetate was completely dropped in the PVA mixture, and the mixture was further stirred for at least an hour for complete evaporation of ethyl acetate. The PVA i am using is High molecular weight

I used a concentration of 0.039–5 mg/mL to determine the MIC for the chitosan solution using the microtitre plate method. The OD value was taken at 630nm. How to calculate MIC90 and MIC50?

What could be the cause of loss of competency in BL21 Competent E. coli cells? I experienced a problem during transformation—the cells were unable to take up the plasmid. The plasmid itself is functional, as it transformed successfully into other competent bacteria. These BL21 cells were stored at −60°C for one month. Could this be the reason for the loss of competency?

The cells are from New England Biolabs (NEB), catalog number C2530H.

Hello all, I'm about to do a sampling of a landfill soil to do the characterization of the possible Mps inside, I follow a sampling method where it will eventually be oven-dried, but I don't think I will do that in the meantime, so would you please enlighten me how to keep the soil sample until further analysis? can I keep it at low temperature or any other suggestions? an answer would means a lot for me, thank you!

Hi everyone,

I am currently working with mouse skin and lung tissues preserved in RNAlater (Invitrogen). However, I have limited experience with single-cell RNA sequencing (scRNAseq). I would like to ask if anyone has experience isolating single cells from RNAlater-fixed tissues. Specifically:

I would greatly appreciate any advice, protocols, or insights you could share regarding this matter.

Thank you so much for your support!

Best regards, Thuong

Need clarification about the capabilities of the Qubit, especially in distinguishing DNA purity from concentration.