Similar systems to Vibratome VT-1000S ?

Date palm micro propagation.

Strains that have polyQ::YFP (AM141, AM101, HA659 and many others) do not fluoresce under yellow light filter, but they do under a green one. Of course green is very close to yellow, and this probably explains why they do fluoresce green, but why not yellow? Someone has the same experience as me?

Hello, everyone. I used a transgenic vector for SCNT and successfully obtained cloned individuals. I screened the cloned individuals for the presence of vector by PCR and distributed individuals into 2 groups: Group 1 containing transgenic vector and Group 2: without transgenic vector (called control). Next, I performed QPCR to evaluate the expression of gene. I found individuals with transgenic vectors (Group 1) also had overexpression of gene. But there were also few individuals in group 1 that although contains a transgenic vector but the expression of the specific gene was similar to the control group (without vector). So, need an expert opinion that why the few individuals who presented normal gene expression was with transgenic vector?

Thanking you in Advance.

Kind Regards

We are considering different brands of stereo microscope to set up a small C. elegans laboratory. Looking for recommendations and any general advice on microscope features that you feel are important for C. elegans culture and research.

Features I expect are critical in deciding on the microscope we choose:

- Micrometer eye lenses - helpful for selecting/screening larval stages by size?

- Fluorescence capability - we won't need this at the start for wild-type strains, but I expect this is going to become a critical feature for transgenics. Have looked at the Nightsea fluorescence adapter, but leaning more towards an integrated system.

- Optical quality - we are looking into live worm tracking software - Is this possible with cheaper stereo microscopes or does the quality of the optics play a critical role in the software tracking accuracy?

- Ergonomics - C. elegans culture requires a lot of time on the scope - what precautions do you take for reducing neck and eye strain (e.g. is it best to have a scope with adjustable/tilting head?)

Thanks for your responses!

Use of auxotrophic mutants lines for transformation of fungi is a traditional practise but it does not enable future discrimination between the wild type and transgenic line. Could basta gene serve to enable selection of the transgenic line from the wild type or from the back mutants of the transgenic line?

I got first generation of transgenic line, and did PCR screening. but when i inoculated PCR positive seeds to antibiotic plates for segregation analysis, I am not getting the mendelian Ratio. Can anyone please suggest me, what to do and how could I screen the transgenic plants?

Whether the transgenic DNA fragments from the feed can enter the aquatic environment through the excretion of aquatic animals ?

There are only few reports on the development of transgenic and tissue culture manipulations in indica rice. What could be the genetic, molecular, physiological reasons? which make indica cultivars to be more recalcitrant to tissue culture regeneration and transgenic developments.