So, you mean it's ok if we use spraying reagent on TLC plate F254 to visualize the compound?
@Nor Syafirah Zambry
It is perfectly OK to use spray reagents on F254 plates. There might be some exception (a chemically specific reagent of some sort), but I've used it with the common ones (sulfuric acid, water, molybdate spray reagent, and iodine chembers too) with no issues. I've also used some of the chemically specific reagents for alcohols and other functional groups too, without issues.
Thank you Jack Silver for sharing your experience and knowledge.
Dr. Harisingh Gour University
Maliba Pharmacy College
Carla W Sabandar
National University of Malaysia
Nagar D P
Defence Research and Development Organisation
Central University of South Bihar
Egyptian Russian University
National Institute of Technology Calicut
Universität des Saarlandes
Nor Syafirah Zambry
Universiti Sains Malaysia