Project/Experimental assistance ？?

I have decided to focus on Integrated Pest Management (IPM) and explore non-lethal methods for controlling pests, without focusing on molecular-level studies. Do you have any suggestions or know of any specific challenges in this area that I could address through my research or pest species that I could work on?

I need the PSD plot code for LFP signal.

I am fresh electrophysiologist in brain slices. I have worked only on extracellular signal detection and patch-clamp combination with voltage imaging in a dish before.

I have recently joined group that is working on neurons in preoptic area near hypocampus. Electrophysiologists working in this group left more then year ago and I am trying to repeat their experiments.

It took some time, but I stated to get relatively good mice brain slices. On other hand, my neurons are dead within 1hr. I am trying to patch more roudish, smooth membrane cells which do not have such visually exposed nucleos. For the first 10-20min neurons has nice spontaneous activity, but after 30min neurons become depolarized. If I keep cell at hyperpolarized stage, then I can evoke action potentials which amplitude overshoots 0mV.

Majority of cells has stable spontaneous activity if I keep cell hyperpolarized. Depolarized cells has resting potential about -35 to -25mV, all membrane parameters looks fine. Pipette offset a bit high (about 50mV), but its just because i am using LowCl intracellular solution with AgCl electrode inside.

I cleaned all set up few times, increased speed of brain preparation, checked pH and osmalarity (extracellular solution 300-305mOsm, intracellular solution calibrated just before experiment to have 11-15mOsm lower then extracellular).

Brain slices kept in my recording solution for 2-3hr looks relatively fine, cells do not shrink or expand. I have to admit that all cells looks a bit roundish after 1hr.

Based on observation cells looks intact and healthy, just these cells ''give up on life''. I am constantly giving carbogen to brain slices. Slicing and recording solutions made weekly. Digitized calibrated few weeks ago.

I will accept any suggestions.

Dear community:

I would like to seek opinions from you on current-clamp mode in electrophysiology. I am trying to record voltage response from neurons, so I first do whole-cell recordings on the neurons and then use the current clamp mode provided by the amplifier (Axon Multiclamp 700B).

If my basic knowledge on electrophysiology is correct, then doing whole-cell recording basically "open a hole" on the cell membrane. This may effectively change the electrical structure of the cell, and cause distortion of voltage signals sensed by electrode. In particular, I wonder if whole-cell recording by itself will decrease the input resistance of the cell, so the current will "leak out" from the hole we make on the membrane, instead of charging the membrane. This problem, if true, will be particularly bad for me, because the neurons I am interested are peripheral sensory neurons, and are small (soma <15 micron in diameter, total capacitance <15 pF). A 2 micron hole on the soma will really change a lot in these neurons.

Any feedback or opinions are appreciated. Thank you.

I'm working with F11 cells (a DRG Hybridome cell line), and I'm trying to do current-clamp on this cells but I only can register RMP ~30mV, and this cells only spike a single action potencial. I don't know how I can solve this question. How can I work on the RMP value? And how can I increse the excitability of my cells?

Hi all,

im working on investigating epigenetic changes to a presynaptic transmembrane protein that seems to be important in PV neurons. I want to investigate neuron functional difference associated with changes in these proteins. Is there a way to select for parv albumin neurons specifically to then patch clamp and measure their activity?

Thanks in advance!

Hi

I used Fluo-4 based live calcium imaging techniques, now I have results in the form of video, but I don't know how to analyse the result.

Can anybody help me with it?

is there any simple model or empirical equation to assess the fate of pesticide in area where there is no previously recorded data