Need a image database of any fruit ?

Dear All,

Please help identify the possible symptom observed in Capsicum annuum leaves. From my observation, it doesn't look like it is pathological (my guess is a physiological symptom but i'm not sure of which).

Thank you.

There's no fungi or bacterial infections

simulate the system's physical layer performance including data generating, source coding ,channel coding , modulation and multiplixing?

Kindly assist me with suggestions on how to dissolve total proteins (from fruits) easily using appropriate buffers. I used resuspension buffer containing 0.5% SDS, 50 mM Tris-Cl and 100mM DTT but i still find it difficult to dissolve the pellets i obtained after precipitation, and washing with 80% acetone, resulting in major loss of proteins. I would be grateful. Thank you!

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I used DTT in protein extraction for WB, but unfortunately it was stored at RT when in the label the storage temperature was 2-6°C. What are the consequences? What is DTT's role in protein extraction before WB? I got only one band after electrophoresis, do you think it's DTT's fault?

Now I have put DTT in the fridge for next extractions but I guess that is too late

Thanks for your help

Hello to whomever is reading this.

I am working on HPLC analysis of anthocyanins in sweet potato leaves and tubers.

One of the methods I have found requires a mobile phase solvent of "5% formic acid in acetonitrile/water 1:1 v:v".

Do they mean 5% formic acid in acetonitrile 1:1 v:v?

Also, how do I prepare 5% formic acid from 98-100% formic acid? Do I just use C₁V₁ = C₂V₂?

Thank you for your help!

Hi,

Whenever used Scikit-learn algorithm (sklearn.model_selection.train_test_split), is recommended to used the parameter ( random_state=42) to produce the same results across a different run.

why we used the integer (42)?

can we use another number?

thanks

i injected immunized hemolymph of larvae into FPLC (fast protein liquid chromatography)

to separate peptides from each other.

every peak of protein separated in a falcon tube in 5 ml of Sodium chloride buffer.

next step is lypholization , after samples were lypholized , protein powder plus sodium chloride crystal is precipitated .

should i do desalting for protein to have a pure protein or can i use it as a whole for inhibition assay fro bacteria??