Is tonsillectomy has significant effect on the immune system?

Hello! I am trying to determine the best controls for my western experiment. I was wondering what the best controls are with a small kDa size?

After infecting HT1080 cells with shScr, shCol18a1#3, and shCol18a1#4 viruses made from the pKLO.1 vector and selecting with puromycin, I will seed them in preparation for a proliferation and migration assay.

For the proliferation assay, I will seed 40,000 HT1080 cells in three 6cm plates for each sample, resulting in nine total plates. I will count one plate of each sample on days 4, 5, and 6 to get the growth curve.

For the migration assay, I will need a single-cell confluent layer for a scratch test, or wound healing assay. I will use a 12-well plate, leaving several wells for each sample. I will vary the densities between the wells, ranging from 250,000 to 500,000 cells. Thus, I can begin the assay as soon as the cells adhere to the plate.

Are these seeding densities reasonable for my experiment? I appreciate any advice.

Hello, I am setting up experiments with inducible ERT2-Cre mice, where our control mice received vehicle only (peanut oil). Do we need to put the controls and TAM-treated (0.1 or 1 mg per day, i.p, ,for 5 consecutive days) mice in separate cages? I am wondering if they are co-housed, and the mice eat the feces, if enough TAM can be ingested by the control mice to induce Cre expression. Thanks.

We know that our star, the Sun loses about 10^-14 of its mass per year as a result of electromagnetic radiation and particle emission. That reduction in mass should show up as a decreasing gravitational red shift. Same thing should happen to entire galaxies. But isn't it true that the galaxies we observe that are farther from Earth are also the younger we see (because light has taken millions of years more to come to us) and, as a consequence the more massive when we consider entire galaxies? (Because we cannot possibly see them as they are, but as they were millions of years ago.) Shouldn't we expect, correspondingly that the gravitational red shift of an observed galaxy will increase with its distance to Earth?

I am a Research Scholar from INDIA. Doing my research in Human Resource Management.

I am currently working on a research about the Manager's Emotional Intelligence influences the Service employee's performance. Where;

Manager's Emotional Intelligence (MEI) - 1 Dependent Variable - Qualitative Interview and Quantitative Survey

Qualitative Interviews from Managers and Perception Based Survey from the service employees about MEI. (Data Triangulation)

Service Employee's Performance (SEP) - 7 Independent Variables - Quantitative Survey

The SEP is measured from:

7 Variables of SEP from Customers as a Quantitative Survey.

Could someone help me out?

Are there any high-impact papers in top journals (Cell, Nature, Science publications) that show the possibility of mimicking mechanical stimulation tissue responses such as skin growth and muscle hypertrophy via drugs?

In our cell culture lab, the incubator has a problem with making CO2, and all our cells are in there. Do you have any idea to make CO2 in the incubator or any idea about protecting our cells while we fix our incubator.