Is it possible to do flow cytometry on frozen/thawed PBMCs?

I wonder if I can thaw a frozen PBMCs from nitrogen phase, wash them and surface or fix/perm and internally immunostain them and test them with flow cytometry? I tried to do surface staining on frozen/thawed/wahsed PBMCs, but they all were dead or (not recovered).

Does any one have a tested protocol?.