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Question
Asked 30 June 2014

Is it okay to use ciprofloxacin in cell culture media as an antibiotic?

I work with drugs with anticancer activity, currently I am culturing A549 cells. Would it be ok for me to use ciprofloxacin as an antibiotic as it has chemotherapeutic activity? What should be the concentration that will prevent it interference with my drugs and at the same time will maintain its antibiotic effect?
Dear Saba,
in case that your antibiotic interferes with the experiment, you could leave it out in the actual experimental design, e.g. when seeding the cells into the multi-well-plates. Then you have a higher risk of contamination, but if you work in a clean way, not very often.
Good luck!
Christoph Leder