Asked 5th Jul, 2023

Is it alright to use a solvent to dissolve the polymer that is different to the eluent of the GPC machine?

Hello, I am trying to run GPC on my samples. However, the GPC available in our lab use THF as an eluent.
My polymer only partially dissolves in THF, but fully dissolve in Chloroform. Hence, I want to ask is it alright if I dissolve my polymer in Chloroform and run it on THF-eluent GPC?
Many Thanks in advance!

All Answers (3)

Alireza Shaabani
Shahid Beheshti University
Yes, this is possible, but you should ask the column manufacturer if it is possible to use another solvent in this column. If the column is not supported, it is possible to damage the column. Still, there is another problem! you should also calibrate the GPC with chloroform solvent!
James E Hanson
Seton Hall University
I don’t recommend this - there is a good chance your polymer will precipitate in the system - if you are lucky it just mucks up the injection part for you to clean , if not you lose the column.
when you say only partially dissolves - is this low
solubiity (so use samples below the solubility limit) or fractionation - high MW not soluble (which means the GPC will be of a different Material than the solid).
Natchanon Jivakanunt
Imperial College London
Thank you very much for your answer! Basically my polymer is PHB (Polyhydroxybutyrate) which I belive is not soluble in THF. However, I found this paper in which they did dissolve the polymer in Chloroform and used it on a THF-eluent SEC. I did run a few samples already and it seems to me that everything is alright!

Similar questions and discussions

Related Publications

Die hier vorgestellte Arbeit befasst sich mit der Synthese von Halbsandwich-Bis(hydrocarbyl)-Komplexen der Seltenerdmetalle sowie deren Eignung als Prä-Katalysatoren für die koordinative Polymerisation von Isopren. Insbesondere wurde der Einfluss eines N-Donor-Seitenarms an Cyclopentadienyl-Liganden untersucht, der zu einer „Constrained-Geometry“ d...
Full-text available
Resumen En este trabajo se describe la síntesis tanto de copolímeros dibloque poli(isopreno)-b-poli(ε-caprolactona) (PI-b-PCL) como de terpolímeros poliestireno-b-poli(isopreno)-b-poli(ε-caprolactona) (PS-b-PI-b-PCL) y poli(isopreno)-b-poliestireno-b-poli(ε-caprolactona) (PI-b-PS-b-PCL) mediante el empleo de n-BuLi como iniciador y benceno como sol...
Got a technical question?
Get high-quality answers from experts.