Is there any available scientific evidence shows that heavy metals contaminated crops play a role in the incidence of hepatocellular carcinoma (HCC)

Based on published research papers, for inducing hepatocellular carcinoma in mice or rats promoting agents (carbon tetra chloride, phenobarbital) have been used in combination with a prior dose of nitrosamine compounds (eg: N-nitrosdiethylamine). Can anybody explain that why do we need to use promoting agents when nitrosamines itself shows carcinogenicity?

I injected the h-AMSC-luciferase (10 ⁶ ) into the mouse model of hepatocellular carcinoma in situ by tail vein. At the beginning, the h-AMSC can be detected in the lung by the small animal imaging systerm (2 hours after injection), but after 30 hours nothing could be detected! I wanted to know whether the h-AMSC could homing to the tumor that inplanted in the liver. Is anyone had been did this experment? Or give me some suggestion!

I want to know the viral loading condition of QGY7703 cell lines.

In General: propagation of Primary cell culture without using Growth factors.

Please provide with practical details.

What are the recent trends concerning Hepatocellular Carcinoma Prophylaxis & Treatment?

Are there any clinical trials going on??

In Europe please

Regards

EPL

DEN shows liver-specific toxicity, as a result it is mostly used to induce various kinds of liver cancer. But there seem to be a lot of heterogeneity in the method of administration of DEN.

In hepatocellular carcinoma, there are many things that change such as increasing of tumor cells and decreasing of its own cells so what happened to its functions ? For example, detoxification, gluconeogenesis etc. 

Thank you so much