Is there a way to remove cover slips from IHC slides?

I immunostained whole mesenteries from mouse. I mounted them in Dako Fluorescent Mounting Medium. Now I would need to counterstain them. Does anybody has any idea to remove the coverslips from the slides, in keeping the samples safe ?

Thanks a lot !

specially for IF How can I demount the slide using PBS?

After dehydrating and coverslipping, there was some excess permount on the slide.  I removed this using xylene and it worked great but as the xylene dried it has left a small streak on the glass coverslip and distorts the view of the tissue a bit.  How do I get rid of this streak?

Dear friends i have only H&E stained slide and i have to do other special staining from this slide only. kindly suggest me the procedure for complete destaining of this slide.

In our mouse bladder and liver sections we see a great deal of folding (creases in the tissue coming out of plane from the slide). May be noticed as early as upon transfer to water bath: tissues of liver section appear to wrinkle or crease almost like shrinking (43C DI water). Next day resting slide for 10 minutes at 65C does not seem to significantly decrease folding.

Sectioning method uses paraffin block chilled in freezer and regularly chilled with ice pack. Regardless of new or worn blade portion.

So adding mounting medium and then placing the coverslip onto the slide sounds and looks easy enough, but there's still so many things that could go wrong. For one, I just can't seem to be able to completely avoid the air bubbles which is really frustrating when your trying to get a good image and there's a small bubble right in the area of interest. Does anyone have some good advice for this last step in the IHC process? All kinds of tips and tricks are welcome. My spinal cord tissue are mounted on slides and my brain sections are free-floating, but both seem to have the same with air bubbles.

In a lab I rotated in, we always reused primary antibody but never reused secondary. In the lab I've chosen to remain in, it is considered okay to reuse secondary antibodies. I am a bit hesitant to do this. We store the antibody cocktail in PBST in 4 degree refrigeration, and the secondary is wrapped in foil.

The other question on research gate concerning this is specific to Westerns, and I'm concerned specifically with immuno.

Dear All,

I recently started doing immunocytochemistry/immunofluorescence and I found that removing/picking up coverslip from 24-well cultured plate is very difficult, before I can place it to a microscope slide. Could you kindly share any tip/trick to remove coverslip from 24-well cultered plate?

Thank you very much

Cheerio,

Is there a way to rescue my tissue sections after they have dried out during primary antibody incubation overnight? If I boil the slides in antigen retrieval solution and repeat the staining procedure will I still have spurious staining?