Is there a way to determine the quality of MUF labled substrates?

As a part of my MSc by Research we will be utilising high throughput microplate assays following Jackson et al. (2013), a technique that utilises 4-methylumbelliferone (MUF) labled substrates.

In our lab we have some of the necessary model substrates ( 4-MUF-β-D-glucopyranoside and 4-MUF-N-acetyl-β-D-glucosaminide) but their condition is unknown.

Does anybody know if there is a way to determine if they are still fit for purpose as they are expensive to purchase and it seems like a waste of money to purchase more if they are still good to use.

Thanks in advance for the help.

Enzyme Activity Assay

Fluorometry

Soil Analysis

Extracellular Enzymes

High-Throughput Screening Assays

22nd Nov, 2017

Lukas Kohl

University of Eastern Finland

The answers above are great, and I agree that your compounds are likely still good for use.

Still: Buy new ones.

The time you/your student work on establishing that they're still good will cost more that re-ordering the labelled substrates, and it's one less issue that could come up during review when you get your results published.