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Asked 13th Nov, 2017

Is there a way to determine the quality of MUF labled substrates?

As a part of my MSc by Research we will be utilising high throughput microplate assays following Jackson et al. (2013), a technique that utilises 4-methylumbelliferone (MUF) labled substrates.
In our lab we have some of the necessary model substrates ( 4-MUF-β-D-glucopyranoside and 4-MUF-N-acetyl-β-D-glucosaminide) but their condition is unknown.
Does anybody know if there is a way to determine if they are still fit for purpose as they are expensive to purchase and it seems like a waste of money to purchase more if they are still good to use.
Thanks in advance for the help.
22nd Nov, 2017
Lukas Kohl
University of Eastern Finland
The answers above are great, and I agree that your compounds are likely still good for use.
Still: Buy new ones.
The time you/your student work on establishing that they're still good will cost more that re-ordering the labelled substrates, and it's one less issue that could come up during review when you get your results published.