Is there a way to determine the quality of MUF labled substrates?
As a part of my MSc by Research we will be utilising high throughput microplate assays following Jackson et al. (2013), a technique that utilises 4-methylumbelliferone (MUF) labled substrates.
In our lab we have some of the necessary model substrates ( 4-MUF-β-D-glucopyranoside and 4-MUF-N-acetyl-β-D-glucosaminide) but their condition is unknown.
Does anybody know if there is a way to determine if they are still fit for purpose as they are expensive to purchase and it seems like a waste of money to purchase more if they are still good to use.
Thanks in advance for the help.