In Gram staining, what are the tiny dark purple dots inside or outside the bacterial cells?

we are preparing to submit a manuscript in field of plant science. However looking at our current budget we realize we won't be able to afford the common $2000 processing fees charged by most open access journals (all our targeting journals :<). I wonder if there are ways like special searching service that can find suitable free-to-publish journals, either open access or "society" journals?

or can anyone recommend some free-to-publish journals in the field of plant science/biology of IF around 3-6?

On the other hand, is there any other possible solution to publish a manuscript with relatively low cost but without compromising the quality of journal too much? 

EDIT: thanks for some great insight. the manuscript is focused on plant agrobacterium transient expression systems and plant parthenogenesis-related proteins and responses. some of the key aspects will be plant biotechnology and plant-pathogen interactions. We originally targeted plant biotech J and frontier in plant science and etc., but all charged pretty steep.  . If any one can recommend a Free-To-Publish journal with relevant scope, will be greatly appreciated!

Today i received a GAPDH- AB, MdCv-F and SNV-LTR-F/R primers forward and reverse and this is the first time for me to make a PCR reaction and I think I need to prepare a  100 µM stock solution from these primers but i did not receive any data sheet to explain the amount of water to add to the primers so I need to know how to know the amount of water to be added to these primers.

This is to understand on how the physicians or microbiologists and other related health workers respond to such unprecedented situations.

Dear Research gate,

I just found that our unpublished manuscript is in the pre print. I am wondering why that title can be obtained by ResearchGate as well as our manuscript. Since we are still working on it . Please kindly let us know how to remove it and why it can be there whithout my consent as corresponding author. Even only title I think it should not be there. Thank you

here is the title: Microbial diversity of Indonesian kefir grains and development of kefir

2 added with probiotic Lactiplantibacillus plantarum subsp. plantarum Dad-

3 13

Hi anyone who reads this, I am a MRes student looking at antibiotic application on S. aureus biofilms. After disrupting my biofilm to quantify antibiotic application on TSA. I re exposed the same disrupted bacteria to TSB on a 96 well plate. The TSA plate quantified growth, whilst the TSB plate showed a lack of growth at some antibiotic concentrations that had grown on TSA. When re exposing the plated bacteria back to TSA there was a lack of growth.

As i tried to grow vibrio using LB agar media, and the whole lawn of vibrio was there after the incubation of 12 hrs at 37 degree. And the colonies were overlapping. Suggest the way to get clear colonies on the plates.

Basically, I would like to quantitatively detect total bacteria in mice feces. How can I obtain a standard curve to reveal total bacteria quantitatively?

By the way, I have one bacterial species that I grew in a suitable medium, and I obtained a standard curve by making serial dilutions, and I found that bacteria in the DNA whose amount I did not know by substituting it in the Ct equation (obtained from the standard curve). But I don't know how to quantify total bacteria. I would be glad if you help.

MICRO INQUIRY