Congratulations! After establishing a new cell line, proper cell line characterization is crucial. Extensive cell line authentication, including phenotypic or genotypic identity testing is required for the newly established cell line.
The origin and history of the cell line, cellular morphology and growth characteristics, proliferation ability, cellular identity ( DNA fingerprinting/STR karyology), purity of the cell line (namely, absence of contaminating cells, microbial contaminations, and contaminations by adventitious viruses) and genetic stability (gene copy number by qPCR/STR) need to be established using various cell line authentication methods (assays).
This information about the cell line will be required if you are going to publish this cell line. This will also help others in the research field to select your cell line as an invitro model system for study.
I think first you will have to ascertain that the Cell line is actually "cell line" and not just transformed cells. You may go through literature to achieve and prove it. Also you will have to / or must have done some in vitro work, where that cell line is being utilised!! Publish those results and defend that as a cell line. Participate in some conferences and present posters or seminars to make it popular. Also donate the same to young researchers and also you will have to make a huge stock of it...if its so interesting and useful. Get some funding from private or government agencies...justifying the importance of the cell line.
Overall its a long process!!
Also, submit a stock in national repositories...like NCCS or ATCC or ECACC. they will guide you maximum on that.
This SOP is for the routine maintenance and expansion of adherent cancer cell lines. It also details how cells are harvested and counted for use in downstream protocols. This protocol is also used in expasion of Cas9 transduced cell lines, prior to banking. Process diagram: