I am interested in your work, I am studying retina degeneration and OPA1 protein

We are planning to study the effect of some compounds on mitochondrial function in retinal photoreceptor cells. In vivo experiments showed that these compounds are effective on rod photoreceptors.

We plan to use retina photoreceptor cells in culture.

Do you know which cells with rod markers would be the best to use?

Hello everyone,

I have a pair of fixed zebrafish's heads (PFA 4%) and I need to know if there'd be any problem using them to study the dopaminergic neurons of the retina.

I read a lot about the technical issues of the detachment of the retina and I don't know if it's worthy the idea of working with these fishes.

Best regards.

If yes, when do you start to see the effect? Within hours of mounting or several days? Is it case specific?

I have had nice stainings with several synaptic proteins/markers when using AF647 in the mouse whole-mount retina. In all cases, I used Vectashield. However, it seems to particularly quench AF647-conjugated Streptavidin.

Hello everyone,

I am trying to visualise retinal ganglion cells in horse whole-mounted retina with a Nissl-staining. However, a peculiar effect occurs when I try to mount the tissue with Entellan New, from the moment I put a drop on the tissue it appears to dry out completely (the pictures were taken moments apart). When the slide is put back into xylene it returns to normal, retrying to mount produces the same drying effect.

The problem does not seem to be with the Entellan, since it works perfectly for Nissl-stained sections.

I did not find any record of this phenomenon anywhere which is why I wanted to ask if anyone has ever experienced something similar or knows why it happens?

Thank you!

Hi all, I am new to RNAseq analysis and am using GSEA to start with. I have watched Katherine West's tutorial video which was very helpful, but I am trying to figure out which chip platform I need to select as the data I received back from the genomic research facility uses different IDs than the ensemble IDs in Katherine's sample data set. I have listed a sample of the IDs below. The data is from microglia isolated from mouse retina. Thank you very much!

Lag3 Chst2 Idua Gpr162 Fblim1 Lox Stk11ip Gpnmb Zbtb4 Tmem8b Sgsh Rogdi Rxra Cyb561a3 Slc46a3 Klhl24 Katnip Peli2 Tbc1d10a Atp13a2 Fbxo32

Affected fish revealed some clinical signs as follows:

Clinical signs related to inflammatory lesions in the CNS, including the brain, spinal cord and retina. Fish lose their appetite and their growth rate is reduced. Fish may be seen whirling and darting in affected fry in the tank in an abnormal manner, as they lose their sight and motor control. They are found either at the top or bottom of the tank as the ability to control their swim bladder is lost. Changes in coloration may also be observed, with species either becoming darker or lighter in color (In affected grouper would be darker and in Barramundi fish it could be lighter).

Here are some pictures about histopathology of Retina and Brain tissues and external signs.

What do you think about mentioned disease and did you have an official report in your country?

Please I need some help with possible differential diagnoses and/or management plan. Fundus photo of an active 47year old male African, Right eye. VA=CF,  exotropia approx. 30^o. Lens, cornea, vitreous are all normal. Good pupillary reaction with mild RAPD, IOP 14mmHg. History of decreased vision since childhood. No history of trauma, diabetes, HIV, or hypertension. The left eye is normal. 

I'm trying to implement retina filter implementation that operates on the principle of human vision. Therefore they are several filters which recognize contours and movements in image sequences.

One of them is called OPL filter and has the  transfer function in the paper attached:

Is there any possibility to find an appropriate filter mask of filter kernal that represent this transfer function in the paper attached.and please also how I may apply this transfer function, i.e, on one image or image sequence.

and also how can i use temporal derivation. Thanks

Please check the paper attached.

Any details about retinal causes of amblyopia or results suggest a relation between retina and amblyopia would be of great help.