I am interested in developing a simple circulation module using Microsoft access 2007 for use in a library setting, can anyone help me?

Please give your valuable opinions.....

Library Automation with Koha and Institutional Repository with DSpace Implemented in a library. If librarian integrated VuFind software with those (koha and Dspace) for the search and browse the library resources (OPAC and institutional authors publications). Also Considering as discovery tool in the library. Apart from that if any features.....

To all the brilliant scientists out there,

Recently I've been performing single cell DNA library for NGS illumina, and there was an issue that bothers me for weeks, I wonder has anyone encountered the same situation or understand what is the problem.

When I checked my library size on a Bioanalyzer, I noticed there were adapter dimers, so I tried a reduced AMPure XP bead ratio (from the recommended 0.8X twice to 0.7X twice) for library clean-up, and I found I can almost remove all the empty adapters.

However when I analyzed sequencing data from these 2 library: 0.8X twice VS 0.7X twice, I found the mapping rate was 40% VS 10%, I wondered if this is because I reduced the bead ratio, which cause the loss of a good proportion of my fragment?

My expected length is ≥ 300 bp.

Thank you so much for all your help!

What if we merge these two different software's rather than using them separately. Kindly suggest if we can make it possible and how ?

I want to design TPMS Lattice present in nTop library of different pore size and porosity. What are the steps to do this?

I am working on a ddRAD-seq experiment and looking for a detailed protocol, particularly focusing on restriction enzyme selection, library preparation, and adapter selection. I would appreciate if anyone could share an optimized protocol or insights on choosing the best restriction enzymes for different genomes. Additionally, any recommendations on adapter design and ligation efficiency would be helpful.

Has anyone encountered challenges in enzyme compatibility or library preparation steps? Any troubleshooting tips would also be valuable.

Hello everyone, We have done library preparation for Genotyping-by-Sequencing (GBS). The sample source is gDNA from chili leaves. The restriction enzymes are using EcoR1 dan Mse1. We've designed the common adapter that compatible with overhang restriction site and universal adapter for Illumina sequencing. Then we'use DNA/RNA UDI index illumina. In sequencing procees final library from GBS, we pool with final library of amplicon and mRNA. We use PE 150 for sequencing. Unfortunately, we didn't get any reads after sequencing.

Could you give us any recomendation how to optimize the library preparation and sequencing to get the optimal output of sequencing.

Your suggestion is very helpful