How to write BID as a total value for one day?

For example, to extract and cluster patterns of documents according to specific criteria or to extract similarity criteria from a collection of documents, etc.

Greetings all, Our team, '#THE GLOBEST TEAM,’ presents a #great opportunity to participate in several in several #American dataset clinical studies related to the #internal medicine field. Your mission is to #write a specific section of the article and we will review your work. Once we have reviewed the manuscript, you will make any necessary edits related to your mission. If you have participated in #10 original research studies or reviews previously, and you are a #well-scientific writer, please leave a comment with your #name, #Google Scholar account, and #email address. #Research #opportunity #clinicalresearch #USAresearch #National Center for Health Statistics #THE GLOBEST TEAM

How to monitor the activity of the extract if solvent also has antibacterial effect

Hi , i want to publish my Research Article subject is Pharmacy and pharmaceutical sciences

topic is antimicrobials

Thanks

Hi,

I need to perform a microdilution agar assay to determine MIC values for some essential oils according to the method described by Golus Et al., 2016 (DOI: 10.1111/jam.13253) which is a modified method of CLSI (2015). They suggest netting 100 µL consisting of approximately 10 µL of essential oil and 90 µL of molten agar media and below 2% DMSO and then filling each well of the microplate with 100 µL of the mixture. Then each well would be inoculated with 10^4 CFU. I guess they have used nearly 200 µL of essential oil + medium in the first well, right? Or else how is it possible to use an initial 100 µL and be able to dilute it in wells while having a final volume of 100 µL per well?

1. If I use less medium, say 40 µL of medium with 10 µL of essential oil to obtain near 20% concentration then inoculate with 10^4 CFU, will the growth of microbes alter significantly? If yes, does it even notably affect the results of MIC?

2. What about using a more medium to essential oil ratio?

3. Can I reduce the amount of inoculum the same ratio as the medium volume has been reduced in respect of the reference method to prevent any adverse effects?

I appreciate any suggestions or answers.

Sincerely, Nafis

There is an increasing concern about non-communicable diseases. How can we (clinicians and researchers) bring change and facilitate people through digital applications and software? How can we conduct a research based on this specially in Low Middle Income countries?

How long does it take for Annals of Internal Medicine: Clinical Cases (AIMCC) to publish an article in-press? Our team article was accepted and moved to the in-press phase for more than 20 days.

Hi,

In running bacterial MIC with antibiotics, do we actually need to measure OD values in spectrophotometer and after getting values at particular emission and excitation wavelength how we get the final output?

Thanks.