Question
Asked 2 July 2024
How to load Remedesivir drug into mesoporous nanoparticles?
Hello,
I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its loading.
1- Should it be loaded to at the last step of MSN synthesis. Problem with this MSNs are extracted later on through reflux and 60 degree temperature is required. It degrades drug.
2- Should it be loaded after extraction making an ice bath but how long it should be kept on stirring?
3- Later I want to coat it with chitosan MSN loaded Remedesivir and then coating with chitosan. I am concerned about leaching and degradation of drug. Should i load drug first and then add drug?
Thank you for your suggestions.
All Answers (3)
Loading Remdesivir into mesoporous nanoparticles involves several steps, ensuring the drug is effectively encapsulated and released in a controlled manner. Here is a general procedure:
Materials Needed:
- Remdesivir
- Mesoporous nanoparticles (e.g., silica nanoparticles)
- Solvent (e.g., ethanol or water)
- Ultrasonicator
- Centrifuge
Procedure:
- Preparation of Nanoparticles:Ensure the mesoporous nanoparticles are synthesized and characterized for appropriate pore size and surface area.
- Dissolution of Remdesivir:Dissolve Remdesivir in a suitable solvent, typically ethanol, to achieve a homogenous solution.
- Drug Loading:Add the dissolved Remdesivir solution to the mesoporous nanoparticles. Mix thoroughly and sonicate the mixture for a specific duration (e.g., 30 minutes) to enhance drug loading into the pores.
- Solvent Removal:Remove the solvent by evaporation under reduced pressure using a rotary evaporator or by drying in a vacuum oven at low temperature. Alternatively, centrifuge the mixture to separate the loaded nanoparticles and wash with fresh solvent to remove any unencapsulated drug.
- Characterization:Characterize the drug-loaded nanoparticles using techniques like BET surface area analysis, TEM/SEM imaging, and drug release studies to confirm successful loading and release profile.
Notes:
- Optimize the drug-to-nanoparticle ratio to maximize loading efficiency.
- Ensure uniform dispersion of nanoparticles during the mixing process.
- Conduct stability studies to ensure the drug remains stable within the nanoparticles.
This procedure can be tailored based on specific experimental conditions and desired outcomes.
COMSATS University Islamabad
Idowu Goodnews Oluwaseyi Thanks for your detailed guidance about loading. Another problem that I am facing that this drug degrades quickly. I am concerned about its release as it is temperature-sensitive, even if I keep the dilution of drug (not loaded into MSNs) in the refrigerator at 4 degrees next day I take a UV vis spectral scan there is no peak of drug.
Self-employed
Apparently, the inclusion of Remdesivir in mesoporous nanoparticles, as such or at the stage of their synthesis. is not good idea, due to the instability of R and, possibly, its low bioavailability. Microencapsulation by complex coacervation, with possible involving CD, may be more frutful approach. Good luck!
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