How to explain the acceleration of the progression of desminopathy (myofibrillar myopathy) after suffering Covid-19?

In a patient with desminopathy (mutation Thr341Pro DES in the heterozygous state) with the progression of the disease, we note signs and symptoms that are also characteristic of botulism: bradycardia, arrhythmia, AV blockade, a significant decrease in the average duration of motor unit potentials according to electroneuromyography, paresis and paralysis of the striated muscles, decreased sweating, paresis of the gastrointestinal tract, dry eyes, dry mouth, symmetry of neurological symptoms, hoarseness, impaired visual acuity, doubling of objects occurs, progressive muscle weakness. These signs and symptoms are characteristic of botulism, only when a case of desminopathy is detected, they proceed slowly.

When staining with hematoxylin and eosin of a muscle biopsy from a patient with T341P desminopathy, we observe accumulations of inclusions similar to nuclei (arrows in figures 1 and 2, x280). And outside of these accumulations - adipose tissue, which used to be muscle tissue. There are no such massive accumulations of inclusions in adjacent muscle fibers. We assume that clusters of inclusions are not nuclei? Figure 2 is the inverted figure 1.

Greetings all, I am a fifth-year medical student, and currently trying to find a thesis topic. After medical school, I would like to pursue a career in neurosurgery, and therefore, I would preferably welcome suggestions within this discipline. I have conducted my research, and I have formulated a list of areas I would like to explore further. This includes : Implications of deep brain stimulation within neurosurgery A collaborative approach to neurosurgery involving interventional radiologists Having listed topics I am keen to conduct my thesis on, I would be grateful if anyone could suggest particular questions surrounding these. Although I gravitate toward these topics I am open to any suggestions in regard to good questions for my thesis.

Hi researchers,

I have 2 questions about growing SHSY5Y cells:

1. I am currently growing SHSY5Y cells in culture and just wanted to check with those who work on this cell line. Is the morphology of my cells fine (please refer to attached images)? These are undifferentiated cells and should exhibit more clustering than if they were differentiated with Retinoic acid. However, I still do see neuritic extensions in addition to the epithelial morphology. So I am not sure if the cells are growing as they should be.

I currently maintain the cells with complete DMEM (1X DMEM with glucose, 10% FBS and 1% PenStrep). I usually freeze with 70% complete DMEM, 20% FBS and 10% DMSO. I always perform a cell count before splitting and the viabilities have been above 93%.

2. In addition, sometimes when I do not need the cells for an experiment in the immediate week, I typically seed a very small cell number (~400,000 cells in T75 flask), just changing media once every 3 days. But by the end of one week, I usually find the cell number depleting and media turning cloudy. From what I had researched, it is said that this cell line should never be seeded at such low densities and media should be changed frequently. Is this accurate?

I have been now seeding at higher densities and I don't encounter the issue of the media turning cloudy.

Thank you!!

I used an IHC staining for Pan-cytokeratin to test for metastasis from breast to brain. I am not sure if the only cells found in brain that are stained brown-ish are the tumor cells from breast cancer that metastasized or could be any other type of cell already present in the brain?

Thanks!

(Will a single sentence form from two people talking in succession?)

What would be the implications in terms of neurology, personal distress, memory formation, etc?

It is named after Schrödinger's Cat, a quantum mechanics thought experiment - where the cat is considered to be in an unknown state, described in physics as superposition. Just as the cat's state is unknown, so may be the context of a 'seemingly' coherent communication within a Schrödinger's Meeting. The example above shows, if you cannot distinguish between the two speakers' voices then the fundamental mechanics required for coherent communication have the opportunity to break down or be absent entirely. "Person 1 + 2" does not exist, and did not say "Are you okay, sir." as discussed in the example above, however, it is possible for a listener to hear that artificial sentence and label it as intentional and coherent.

The Schrödinger's Meeting example highlights the importance of stimuli distinguishability in our everyday lives. Investigations into stimuli distinguishability and homogeneity, particularly regarding digital text comprehension, is the groundbreaking work of my team. Further materials and research papers related to the topic can be found on my profile.

Environmental stimuli distinguishability as it pertains to the general public is a massively underexplored area of neurology and information theory. My team and I look forward to hearing others' reasoning regarding the possible effects of a Schrödinger's Meeting and/or the effects of sensory distinguishability on the modern human.

---

Aside from the significant implications on neurology and human health, Schrödinger's Meeting may further prove useful in information science, by helping to answer the question of: What is the exact definition of a "communication"? Has the hybrid "Person 1+2" and their artificial sentence communicated? Many people would be hesitant to define this hybrid-sentence as a communication, however, the hybrid-sentence is certainly intelligible to the listener and therefore communicable. To paraphrase, the current definition of "communication" as described by Oxford is a process involving the transmission of information. By this definition, the hybrid sentence is certainly a communication.

At a glance, this thought experiment may seem pointless. However, it highlights the importance of defining exactly what "communications" are.

In addition to digital text-technology advancement, answer(s) to this question may provide mechanical insight into important properties of large data sets, such as metadata, quantum computation datasets, and image-capture rendering. Additionally, Schrodinger's Meeting may provide insight into composite waveform systems, providing context for deciphering probabilistic rates at which coincidentally-coherent noise may form in a given wave-mixing data set.

To save life in desminopathy, can the body purposefully reduce muscle mass, for example, due to decreased heart function or for another reason?

It is known that when hypothermia, the body sacrifices limbs for survival. Is it possible with desminopathy a similar phenomenon?

Hello to all my fellow Biologists.

I have resorted to posting this question as my last desperate attempt to find a place for myself in the world of biotechnology.

I am a first class student with relatively good grades (GPA 3.77/4.00) in BSc. of Biotechnology (Hons), excellent extra-curriculars and competitions under my belt, 6 months of work as a Field/Research Assistant and 4 years of previous work experience in events management. I have experience in the microbiology, molecular biology, antivirals, nutraceuticals and cell culture disciplines. I have also taken a great liking to scientific communication and create visual content to make biology simpler for the Layman, both bother and in my own time.

Despite all this, I haven't had a single postgraduate application succeed for the last 2 years. And though I do understand there is high competition for available spots, I also wonder what I may be lacking despite some telling me I have an "impressive CV" and can do a direct PhD.

It is unfortunate however that my family is not doing financially well, therefore I can only afford opportunities with a scholarship or that are work/salary-based. Perhaps this narrows available opportunities but regardless, studentship scholarships have very evidently not opted for me, simply because "there were too many applicants this time around". Perhaps lacking funds is not enough of a criteria? (Hint of sarcasm).

Additionally, I was born and raised in the UAE (I do not get citizenship), therefore I am also looking for a potential country to eventually settle down in while doing the work I love.

I would greatly appreciate if anyone would know of opportunities I may be able to apply for like fully funded PhDs, or skilled/summer programs and workshops/internships, or even Research or Lab assistant positions you or someone you know may be looking for, because unfortunately, I'm 2 rejections away from being completely out of options.

I would greatly appreciate any input you may have or can share with me! I have also added my CV for your reference.

I don't want my impression of the field I love to be tainted with nothing but rejections, and to settle for a job outside our field simply because I had no other choice.

I look forward to hearing from you all.

Sincerely,

Zahraa Ozeer

Hi. My only interests are in public and private sector accounting and auditing. No interest in medicine. Please remove the latter from my record. Regards, Patrick Barrett.

ANU, A=Australia.