What if we culture it in MCDB 131 medium?
Thanks for the answers, Ranadevan, Alasdair and Berrin. @BerrinSaracoglu The link you posted is correct, but is not specific. Insulin general actions in mammalian cells does not explain why it should be recommended in the culture of MCF-7, since insulin is not a general recommendation. In the particular case of MCF-7, I have discussed with a few colleagues from Rio de Janeiro University and it seems to be a consensus observation that insulin is not necessary if you supplement with 2% glutamin in a phenol red DMEM low glucose. If your culture is a high glucose DMEM with low glutamin, insulin might make some difference, but the culture is not stable. Labs which have used this glutamin high glucose low DMEM without insulin have been able to easily maintain their MCF-7 cultures. We believe there is a metabolism shift to a less glycolytic state. And it have been also observed in the cellular response to clotrimazole, a work from some colleagues in Rio PMID: 22347377
can anybody tell me which kind of DMEM is better for MCF7 culture? high glucose or low glucose?
Sohelia, I think DMEM/F12 to abolish the estradiol like effect of the phenol red :)
Dear, it is better to use EMEM supplemented with 10 % FBS and Antibiotics (100 U/mL penicillin and 100 U/mL streptomycin), the cells grow very well
We are using RPMI with phenol red with additives of 10% FBS, insulin, P/S, non-essential aminoacids and L-glutamine.
Joy Sebastian Prakash
Chettinad Hospital & Research Institute
Old Dominion University
Tarbiat Modares University
German Cancer Research Center
Vyshnava Satyanarayana Swamy
University of California, Riverside
Aya el garhy
Misr University for Science & Technology
Iran University of Medical Science