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Question
Asked 2 March 2021

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have also observed this problem in MC38 and EG7 tumors. This did not happen to us so often until now. Any suggestion or personal experience that helps us to resolve this issue will be really appreciated. Thanks in advance.
Michaelia Cockshell
Centre for Cancer Biology
For an injection of 1x10e6 B16F10 we found day 12-14 there is occasionally some ulceratiin of tumours but it also when we reach the ethical limits of tumour size and the end of the experiment. This has been the case for these tumours for the last 10 years in the lab even with fresh, newly sourced cells vs old cells frozen for years. If you want to take them out longer then use less cells to start with.