How do i edit away these strange nucleotide letters (N, K, Y, B etc) from a Sanger sequence DNA read?

Hi there!! I want to do quality check some Sanger sequence reads and realized that the reads contain some odd letters (N, K, Y, B etc) different from the normal 4 DNA base letters (ATGC). What can i do to edit away these strange nucleotides represented by the strange letters?

Sanger Sequencing

Nucleotides

DNA Sequencing

Reading

Drug Resistance

Falciparum Malaria

Malarial Cell Culture

Bioinformatics and Computational Biology

Medicinal Chemistry

Computational Chemistry

Sibnarayan Datta

These are not strange nucleotides, but they represent ambiguous peaks at those positions, and polymorphism. Each of these letter specific combinations of signals and are defined in the IUPAC list of ambiguous nucleotides. You need to check these positions in your sequence electrpherograms and confirm. If you have peaks of comparable intensity, you may retain these letters while submitting your sequences to the GenBank. GenBank accept sequences with IUPAC codes. Fllow the link while revising your sequences:

https://www.bioinformatics.org/sms/iupac.html

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