How can lignin, cellulose and hemicellulose be extracted and purified from wheat bran?

I have HPLC with fluorescence detector & I want to quantify amino acids of some cereal grain by RPHPLC, what is the best method with derivatization technique?

Hi,

I have samples of food waste,  consist of rice and chicken meat waste, . I want to digest the samples to analysis crud protein. Also, I ash some samples before I digest to analyize Ca and P. For cude protein samples,the problems are the samples can not digest completely in spite of I use one tablet, H2O2 and H2so4. the temperture for first hour is 220 C and for the second hour is 410 C.The samples become milky color and still like this even I keep them in digester for 4 to 5 hours. Also, after I ash some samples to Ca and P analysis. The samples  are still black color after I have digested for 10 hours in spit of it should be less than 30 minutes 

the cereal is high in starch.

i'm searching a method for determine Protease activity in cereal, anyone can help me?

At both very high and very low water activities, lipid oxidation rates are high compared to the rate at intermediate water activities. What can explain this trend?

Why functional properties like WAC, OAC, EC, ES, FC, FS of a flour are done?

Could anybody share with me the list of functional properties with which I could determine various types of cereal flours?

Nothing

My hemicellulose extraction step;

1) defatted rice bran

2) destarch by amylase (termamyl)

3) deprotein by NaOH at pH 11 

4) alkali treatment for hemicellulose extraction by 1.25 N of NaOH for 24 hr at room temp. Then the supernatant was adjust to pH 4.8. The resides was not recover. Then 3 volume of EtOH 95% add for precipitation hemicellulose.

5) 3% of extracted hemicellulose of 10 ml mix with endo-xyalanse from Neocallimastix 

Please recommend me, there is any step was wrong or not?