GMQ
Question
Asked 19 October 2023
How can convert micromoles per second per liter to millimoles per gram of dry cell weight per hour?
I need to convert from micromoles per second per liter (µmol s⁻¹ L⁻¹) to millimoles per gram of dry cell weight per hour (mmol gDCW-1h-1)
Thanks!
Most recent answer
An "empirical formula" for a microorganism most of whose molecules are of profoundly greater molecular weight? But there's the "However..."
All Answers (4)
You'll need to know the density of your cells to perform this conversion. You can determine it experimentally using various methods like gravimetric method, by optical density + buoyant density method, by Transmission Electron Microscopy + densitometric image analysis... or try to find the value in a database/texbook. An estimation could be applied, depending on the microorganism/cell type.
Bruce E. Rittmann; Perry L. McCarty. Environmental Biotechnology: Principles and Applications. Empirical Formula for Microbial Cells, Chapter (McGraw-Hill Education: New York, Chicago, San Francisco, Athens, London, Madrid, Mexico City, Milan, New Delhi, Singapore, Sydney, Toronto, 2001). https://www.accessengineeringlibrary.com/content/book/9781260440591/toc-chapter/chapter2/section/section3
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Where can I get sequences to build up a Diamond database?
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Hi,
I am new with Diamond. Diamond seems to be good as a sequence aligner for protein and translated DNA searches.
I only a concern. Where can I download .fasta files to build up a database?
Is the following database the best one? ftp://ftp.ncbi.nlm.nih.gov/blast//db/FASTA/nr.gz
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