Asked 17th Jul, 2017
How can I obtain this cotyledon sections for microscope?
Should I use a microtrome? Can anyone explain me how to prepare this tissue so that I can observe haustoria in plant cells?
Similar questions and discussions
Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?
- Marcia Chavez
I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak signal for lectin. I'm wondering if this happened because the blocking solution ran off the tissue during incubation. Before blocking, I fix my sections in 4% PFA for 10 mins and then do 3x 5 minute PBS washes. Because the slides are still a little wet, I think that's causing the blocking solution to run off. If this is what's happening, could that account for high background and a weak antibody signal?
How can ı protect my tissue on negative control from nonspecific staining at immunohistochemistry?
- Kübra Aksu
My tissue is a mouse tissue and my primary antibody also mouse. I have already tried mouse on mouse kit. ı have still staining at negative control. My tissue have parafin sections.
Can anyone help me solve about this ?
Does anyone have a protocol for tissue clearance for deep confocal imaging of the brain section?
- Maria Gotkiewicz
I am looking for the working protocol, which I could use for clearing the brain section, 105 µm thick (hemisphere).
Without this I am not able to reach deeper into the tissue than 20 µm,with confocal microscope with Airyscan, what is problem here. Important thing is, that our section has GFP produced by animal, so crucial thing is to have GFP signal in cells saved.
Suggested protocols for Thioflavin T staining on free-floating hippocampal tissue sections?
- Nonkululeko Dhlamini
I'm doing immunofluorescence staining, under confocal microscopy, on 30um thick hippocampal tissue from rats. The fresh brains were post-fixed in formaldehyde, rinsed and are currently stored in 30% sucrose with azide. I plan to cryosection the tissue at 30um thickness and antibody stain for microglia and astrocytes using anti-Iba1 and anti-GFAP, respectively.
To confirm amyloid formation, I will co-stain with Thioflavin T.
Can someone kindly assist me with the following:
1) How to make stock solution of Thioflavin T?
2) How do I dilute the stock Thioflavin to a final working concentration of 20Mm?
3) How long should the sections be incubated in ThT before I can rinse?
4) I use TBS as a wash buffer, will it be suitable to rinse sections in TBS following incubation in ThT? Or do I have to rinse with ethanol?
Thank you in advance for your assistance.
Request to examine and confirm some microscopic pictures of Macrophomina phaseolina infected root sections?
- Lucky Duhan
I am working on the fungus Macrophomina phaseolina in the Vigna mungo host. I want to confirm the infection in the plant roots. I am attaching some of the root section pics taken with the help of a light microscope at 40X.
Please examine and confirm the microscopic pics taken by me.
Is there any handbook/video tutorial for learning Immunohistochemistry (IHC) slide analysis/ scoring ?
- Basim M. Jwad
I want to know how to look/methods of(analyze and scoring) in different parts pathological section like stroma, Ductal epithelial, epithelial lining, luminal epithelial etc. in a different pathological tissue slide.
What is the best way to do immunohistochemistry on perfused gut tissue from rat?
- Teresa Burke
We have recently collected perfused whole mount sections of the rat gut, containing the duodenum, ilium and colon. Following perfusion the sections were cut longitudinally but kept all in one piece. They were stored in 4% PFA overnight before being transferred to PBS with 0.03% sodium azide for a few days. We then transferred the sections to PBS with 30% sucrose for 48 hours. Following this the sections were frozen in one piece using OCT.
Can anyone suggest a good way to section this tissue and if there are any antigen retrieval steps which should be performed when doing immunohistochemistry? We are hoping to look for alpha-synuclein.
Tracking fluorescently labelled extracellular vesicles in tissue?
- Sagar Rayamajhi
I have tissue treated with fluorescently labeled EVs. What is the best way to process the tissue for sectioning that can retain the fluorescence?
I have tried cryostat sectioning. But the tissue sections look dirty, not as good as paraffin-embedded sectioning. And paraffin-embedded sectioning needs to go through a harsh environment (high temperature, xylene) that diminishes/quenches the fluorescence.
Any suggestion here? Thanks
The initial concept of the membrane as a structure or interface that separates the cell from its environment—be it some sort of interstitium, as in the case of a tissue, or a suspension mediumsuch as seawater or blood plasma—was, of necessity, a consequence of the primary generalization, usually attributed to Schwann, that the behavior of a tissue,...