Asked 20th Jan, 2014

How can I find a new gene in contig sequences on NCBI?

There is a Protein entry for a gene, but the Organism hasn't been completely sequenced (according to NCBI). So there are only contigs of the WSG online. How can I find the Gene to my protein there, given a Locus tag and the AA Sequence?

All Answers (2)

Berlinda Verdoodt
Ruhr-Universität Bochum
If you have both a protein sequence and partial DNA sequencence to compare it to, try NCBI Blast using the TBLASTN algorithm, which compares aprotein sequence to the translated (all three frames and forward and reverse) nucleotide databases.
Muhammad Sufian
Mirpur University of Science and Technology
Provide us the NCBI GI accession.

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