1st Feb, 2019
Universidad Técnica Particular de Loja
Question
Asked 22nd Jan, 2019
How to confirm the presence of PGPR bacteria in the field trials?
My question is in relation to the paper research: biological control of Heterodera glycines by spore-forming plant growth-promoting rhizobacteria (PGPR) on soybean
Most recent answer
Thanks Hani Antoun!
All Answers (4)
Hi Oscar
Since these authors used mixutres of different strains, one way of noting their presence is to sample the rhizosphere (soil strongly attached to the roots) of inoculated plants and the rhizosphere of the non inoculated control and compare the total number of Gram positive bacteria in both. One can expect that the total number of G+ bacteria in the treated plant will be much higher than in the control. Separating G+ bacteria can be done by heating the first dilution at 50-60C to induce sporulation and then plating on 10% TSA medium. Antibiotic specific to G- bacteria can also be added to TSA medium.
Regards
1 Recommendation
Similar questions and discussions
Related Publications
Bacterial pustule caused by Xanthomonas axonopodis pv. glycines is one of important bacterial diseases of soybean in Indonesia. The development of alternative control of the disease is required to support integrated diseases management for sustainable agriculture. This research was conducted to observe the effect of straw mulch and plant growth pro...
Effects of five PGPR strains and two mixtures of PGPR strains on soybean plant growth and Heterodera glycines population density in the field trials.
(XLSX)
This is a correction to:
Biology and the Statistic Demographic of Aphis glycines Matsumura (Hemiptera: Aphididae) on the Soybean with Plant Growth Promoting Rhizobacteria (PGPR) Treatment. Jurnal Perlindungan Tanaman Indonesia, 24(1), 54‒60. https://doi.org/10.22146/jpti.49846
In Author’s affiliation, typed as:
Hermanu Triwidodo1)*, Anggun Agus...