How can I assess the expression of your GOI in breast cancer using publicly available databases?

Hello,

Do any one know how TCGA database works? any tutorial to show ho to use it and interpret the data?

Also, any idea about any database website that shows the expression level of specific microrna in different human/mouse tissues normal/cancer?

Thanks

I want to test the potency and efficacy of a therapy against cancer in vivo and wish to use Rat-Sprague-Dawley. Please, any suggestions are welcome. Many thanks

We want to know if one protein can be used as a breast cancer biomarker or not. So we'll have to compare normal and patients, and our question is whether we can get samples from patients who received chemotherapy before?

I would like to stably knockdown my GOI in the E0771 cell line (mouse breast cancer), which I will then inject into mice to establish a tumour-bearing model. However, I have never done this before and also don't know a lot about the techniques involved. So far, I figured out that I need to transfect my cells with a viral vector expressing either shRNA or miRNA targeting my GOI, and that the viral vector I use needs to be able to integrate into the host genome.

Is this correct? What would be the best option for me to use? What steps/techniques does this procedure require (to generate a vector like capable of this)?

The vector also needs to have some kind of reporter in as well (eg GFP) because I need to be able to visualize tumour growth and metastasis over time (live animal imaging).

I greatly appreciate your input

I would like to test the effect of Trastuzumab (ROCHE) on BT474 cells, but I have only Herceptin for infuison. Is it possible to use it for cell cultures? 

I have to resusped it in the classic infusion solution, but I do not know how long could be the manteinance in the fridge (4-8 C).

Thank you.

How to induce Breast cancer (MCF7 cell line) model in Nude mice? Are any supplements needed to grow tumor faster?

I've been tasked with figuring out if certain murine cancer cell lines express specific cancer antigens we would like to target. For instance, does the 4T1 breast cancer tumor model express WT-1? Do you have suggestions on the best way to go about researching this? Is there a database I haven't found? Is the best course of action just to google away? I've been at it for a while and was just wondering if any of you have an efficient way to go about this.