Question
Asked 20th Jul, 2011

GC content in DNA!!

Why GC content in DNA sequence is so important? Also its Uses.

Popular Answers (1)

21st Jul, 2011
Richard Hall
Luminex Corporation
G-C base-pairs have stronger interactions (than A-T BPs) arising from their ability to form three hydrogen bonds in water. A-T base-pairing yeilds only two hydrogen bonds. That is why the melting point of double stranded DNA is higher for high G-C content DNA as well as for longer pieces of DNA. High GC content DNA can make it difficult to perform PCR amplification, because it is difficult to design a primer that is long enough to provide great specificity, while maintaining a melting point below the optimal temperature of DNA polymerase. DMSO, Glycerol, Salt or MgCl2 can all be used to perturb the effective melting point.
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All Answers (3)

21st Jul, 2011
Richard Hall
Luminex Corporation
G-C base-pairs have stronger interactions (than A-T BPs) arising from their ability to form three hydrogen bonds in water. A-T base-pairing yeilds only two hydrogen bonds. That is why the melting point of double stranded DNA is higher for high G-C content DNA as well as for longer pieces of DNA. High GC content DNA can make it difficult to perform PCR amplification, because it is difficult to design a primer that is long enough to provide great specificity, while maintaining a melting point below the optimal temperature of DNA polymerase. DMSO, Glycerol, Salt or MgCl2 can all be used to perturb the effective melting point.
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22nd Jul, 2011
Alia Khan
Islamabad Diagnostic Center
I need exact one step protocol of NS5b pcr.plz help me.email it to me on anila.jahangiri@yahoo.com
25th Jul, 2011
Akalesh Kumar Verma
COTTON UNIVERSITY
it is site of rich methylation/acetylation that regulate different gene expression

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