Does anyone of you know about neurons which innervate bilaterally. For example, Olfactory sensory neurons of Drosophila?

Should be the thinnest, but that isn't as trivial as it sounds.

Hi guys, I have observed a strange/exciting phenotypes in my mouse. The cortical neurons are upside down. There are no apical dendrites, and axons are dendrite-like. I attached a reconstructed image. I would like to know if the polarity is reversed (that would be very cool) or the axon is dendritized but could not find good markers for in vivo staining. Any suggestion? What other things you guys want to do with the phenotypes to make a nice story? 

I usually use the ImageJ plugin Simple Neurite Tracer for a manual analysis. But due to the huge number of pictures I would like to know, there is any way to do it automatically (either with SimpleNeuriteTracer or other plugin)?

I have pictures of the axon and I should measure the length of axon and dendrites and the number of dendrites.

Thanks in advance

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization?

A key element to answer this question is to know if, when neurons from a cortical map project their axons to the dendrite of a downstream neuron, they retain any spatial order proportional to their location in the map.

For example, in the cartoon below, the four neurons from a cortical map (in black) project their axons to a downstream neuron's dendrite (in green). The relative spatial position of the synapses (black circles) is proportional to the relative position of the neuron in the map.

I would be very grateful if you could point me to any relevant paper addressing this question, in particular in the cortex of the primate (e.g. axon tracing experiments).

Thanks!

When you have non-spherical animal cells in culture, after you spin them down and re-suspend in media, the cells can look round. I've seen this from my own experience and I have also seen it in published images.

This is the case for cells with processes/axons/dendrites/filopodia such as neurons.

Are filopodia retracted by the centrifugation? Do they break off? Get wrapped around the cell? Is there a paper that shows this?

I would like a broad answer to this question, but specifically I am asking this question in the context of wanting to submit neurons for single cell sequencing. Where do the dendrites go? Do they get in the way?

I am differentiating Neural stem cells into neurons. I have planned to use Tau and Map2 antibodies in western blotting to differentiate axon and dendrite outgrowth. I would like to know any other experiments which could be used to differentiate the axon or dendrite outgrowth of a neuron other than western blot and ICC.

I'm looking for a membrane protein (found in neurons) or other antigen that will provide very strong staining from IHC. I would prefer an antigen that is uniformly expressed across dendritic, axonal, and somatic membranes on the neurons.

We have robust staining for a certain ion channel that is located in the neuropil and wish to know whether its axonal or dendritic. Hoping to use double immunostaining to find out.

I am trying to grow SH-SY5Y neuroblastoma cells. or main objective is to get the dendrite and axon structure and neuronal network like growth. We are getting the small connections between neuron to neuron,but it takes 10 days to get even minor connection. In the complete plate we are getting in some places such growth, where as maximum time we get clump growth. So can any one suggest any technique to get neuronal network based growth?