I thank you all for helping me design my cell viability assay with much more criteria. I will be searching for good references to strengthen your valuable tips for this experiment
It all depends on the cell line you are using. I have tested DMSO toxicity on three prostate cancer cell lines, one cell line shows a statistically significant effect at >=0.5% while the other two are statistically significant at >=1%. You could design an assay using varying concentrations of DMSO to determine a threshold level for your extracts. Good luck :-)
This page might help you :
Typically DMSO <0.01% final concentration should not be toxic to cells. I have worked with 0.01% DMSO and did not find it cytotoxic to T-cells.
Thank you all for the answers !
I have been working with HaCat cells using a final concentration between 0,01 - 0,015% and the cells weren´t affected. Very precious tip about paying attention to the cell line you are working with !
Best regards and good luck to all :)
Just to let you know that I have used 0.1% - 0.15% of DMSO and the HaCat cells behaved well. For sure that I have prepared my DMSO control simultaneously.
Best regards to all
University of Bristol
Kris Cahyo Mulyatno
Institute of Technology, Tralee
University of Alberta
University of Lucknow
Medical College of Wisconsin