Question
Asked 16 June 2014
  • Zoological Society for the Conservation of Species and Populations

Does anyone have experience with DNA-extraction from formalin-stored anuran larvae?

Does the FFPE-kit work with tadpoles?

All Answers (2)

Leonid S. Adonin
Federal State Budget-Financed Educational Institution of Higher Education The Bonch-Bruevich Saint Petersburg State University of Telecommunications
I think, You could try CTAB (Cetyl trimethylammonium bromide) method of DNA isolation!
Arne Schulze
Zoological Society for the Conservation of Species and Populations
Yes, thanks! But the standard CTAB revealed only moderate results. I thought that maybe someone has used e.g., FFPE kits.

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Hello everyone. I represent a small group of students from Maastricht University in the Netherlands. We are currently working on a project regarding toe-tapping behavior in frogs and toads (see https://www.youtube.com/watch?v=gl_A4UosQjw). We are collecting as much information as possible regarding it in order to try to shed some light on this very understudied phenomenon. If you've ever observed it and could spare a few minutes of your time to help us in our research, please fill out our questionnaire or share your knowledge with us. Any input is greatly appreciated.
Thank you for your time and happy herping.✌️

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